PXD079655-1

PXD079655 is an original dataset announced via ProteomeXchange.

Title	Mini-Scale Cheese Peptidomics Reveals Strain-Specific Bitter Peptide Profiles During Cheese Aging
Description	This study investigated the impact of individual lactococcal starter cultures on peptide formation and bitter peptide accumulation during cheese aging using a novel mini-scale cheese production system. Cheeses were produced using single-strain fermentations of Lactococcus lactis and Lactococcus cremoris and aged for 45 and 90 days. Peptide profiles were analyzed using LC-MS/MS-based peptidomics to characterize strain-specific proteolytic patterns and bitter peptide generation from casein proteins. The resulting peptide profiles demonstrated strong strain specificity and reproducibility among replicates. Particular emphasis was placed on bitter peptides derived from β-casein and αS1-casein regions associated with cheese bitterness. Two L. lactis strains, E2-4M12 and E1-6M12, consistently produced lower abundances of bitter peptides despite substantial overall peptide abundance, suggesting unique proteolytic characteristics. Comparative analysis between CEP-positive and CEP-negative strains indicated that CEP genotype alone was not a primary determinant of bitter peptide accumulation. These findings contribute to understanding how starter culture selection influences cheese proteolysis and bitterness development during ripening.
HostingRepository	PRIDE
AnnounceDate	2026-08-01
AnnouncementXML	Submission_2026-08-01_09:03:36.503.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD079655
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Russell Kuhfeld
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	phosphorylated residue; acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-12 18:43:46	ID requested
⏵ 1	2026-08-01 09:03:36	announced

10.6019/PXD079655;

10.1016/J.FOODCHEM.2023.138111;

submitter keyword: Lactococcus lactis, Peptidomics, Casein Proteolysis, ProteomeXchange, Lactococcus cremoris,Cheese, Bitter Peptides, Dairy Proteomics, Cheese Aging, LC-MS/MS

Russell Kuhfeld
contact affiliation	Nutrition Program, School of Nutrition and Public Health, Oregon State University, Corvallis, OR, 97331
contact email	kuhfeldr@oregonstate.edu
lab head
Russell Kuhfeld
contact affiliation	Faculty
contact email	kuhfeldr@oregonstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079655
     1. Label: PRIDE project
     2. Name: Mini-Scale Cheese Peptidomics Reveals Strain-Specific Bitter Peptide Profiles During Cheese Aging