PXD079496-1

PXD079496 is an original dataset announced via ProteomeXchange.

Title	Low-Temperature Plasma-Assisted Hydroxyl Radical Protein Footprinting Coupled with Mass Spectrometry for Structural Analysis of Protein Liquid–Liquid Phase Separation
Description	We developed a “non-denaturing, high-resolution structural method” using low-temperature plasma (LTP)–generated hydroxyl radicals coupled with liquid chromatography–mass spectrometry (LC-MS) to probe conformational changes during protein liquid–liquid phase separation (LLPS). Using Tau protein as a model, we found that this method reveals specific solvent accessibility changes and global conformational unfolding in the terminal regions on both sides of the protein during LLPS without chemical additives or denaturation. This method overcomes incompatibilities between LLPS systems and MS, enabling residue-level structural analysis of dynamic, disordered proteins linked to neurodegenerative diseases.
HostingRepository	iProX
AnnounceDate	2026-06-10
AnnouncementXML	Submission_2026-06-09_20:42:23.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tingting Liang
SpeciesList	scientific name: 大肠杆菌; NCBI TaxID: 562;
ModificationList	acetylated residue; methionine oxidation with neutral loss of 64 Da
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-09 20:42:00	ID requested
⏵ 1	2026-06-09 20:42:24	announced

Dataset with its publication pending

submitter keyword: HRPF, Pin1, CaM, Tau

Gongyu Li
contact affiliation	Nankai University
contact email	ligongyu@nankai.edu.cn
lab head
Tingting Liang
contact affiliation	Nankai University
contact email	2120231095@mail.nankai.edu.cn
dataset submitter

iProX dataset URI