PXD079414-1

PXD079414 is an original dataset announced via ProteomeXchange.

Title	Cell-intrinsic steroidogenesis regulates mast cell function
Description	Steroid hormones are well known regulators of mast cell function, but whether mast cells themselves produce steroids de novo and influences their effector function has remained unknown. Here, we show that mast cells constitutively express the steroidogenic enzyme Cyp11a1 and produce pregnenolone. Cyp11a1 expression was detected across tissue-resident mast cells from multiple organs, was conserved in human mast cells, and was enhanced by IL-3 signalling and after activation. We identified Cyp11a1 as a Gata2-dependent, super-enhancer-associated mast cell gene, placing steroidogenesis within the core mast cell transcriptional programme. Loss of Cyp11a1 altered transcriptomic and proteomic programmes in mast cells, with effects on pathways linked to cell growth, immune signalling, and effector function. Functionally, Cyp11a1-deficient mast cells showed increased degranulation in response to both IgE-dependent and IgE-independent stimulation. In vivo, loss of Cyp11a1 reduced mast cell abundance in several tissues but exacerbated passive cutaneous anaphylaxis. These findings identify cell-intrinsic steroidogenesis as a regulatory pathway that restrains mast cell activation and anaphylactic responses
HostingRepository	PRIDE
AnnounceDate	2026-09-04
AnnouncementXML	Submission_2026-09-04_06:49:41.321.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bidesh Mahata
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-08 16:17:33	ID requested
⏵ 1	2026-09-04 06:49:41	announced

Dataset with its publication pending

submitter keyword: Mast cell degranulation, Cyp11a1,Mast cell, Steroid-signalling,  Anaphylaxis, Steroidogenesis, Pregnenolone

Bidesh Mahata
contact affiliation	Group Leader (CRUK Career Development Fellow)
contact email	bm562@cam.ac.uk
lab head
Bidesh Mahata
contact affiliation	Research group Leader, Department of Pathology, University of Cambridge
contact email	bm562@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079414
     1. Label: PRIDE project
     2. Name: Cell-intrinsic steroidogenesis regulates mast cell function