⮝ Full datasets listing
PXD079384-1
PXD079384 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cryo-EM structure of African horse sickness virus VP2 receptor-binding protein enables nanoparticle vaccine design |
| Description | African horse sickness virus (AHSV) is a lethal equine pathogen with no licensed vaccine other than vaccines containing attenuated virus, which pose safety risks. Endemic to sub-Saharan Africa, AHSV has caused epizootics in Spain and Portugal, Cyprus, Morocco, the Middle East, India and Pakistan and, most recently, Thailand. Here, we resolve the 3.11 A cryo-EM structure of full-length VP2 from AHSV serotype 4, adopting its native triskelion architecture and shedding light on a previously undescribed alpha-helical domain anchoring the triskelion core, which is absent in other structurally characterized orbiviruses. Structure-guided mapping identified a subdomain of VP2 as a key target of neutralizing antibodies. Displayed on nanoparticles using the SpyCatcher/SpyTag technology, the newly identified domain conferred complete protection from clinical disease after viral challenge infection in mice and elicited robust and long-lasting immune responses in horses, the target species of AHSV. These findings provide a structural blueprint for the next generation of recombinant vaccines against AHSV and related orbiviruses. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-19 |
| AnnouncementXML | Submission_2026-06-19_02:12:29.060.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mikel Azkargorta |
| SpeciesList | scientific name: African horse sickness virus; NCBI TaxID: 40050; |
| ModificationList | unknown modification |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-08 02:17:31 | ID requested | |
| ⏵ 1 | 2026-06-19 02:12:29 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: AHSV, cryo-EM, Glycoproteomics, DatasetType:Proteomics |
Contact List
| Mikel Azkargorta | |
|---|---|
| contact affiliation | CIC bioGUNE |
| contact email | mazkargorta@cicbiogune.es |
| lab head | |
| Mikel Azkargorta | |
| contact affiliation | CIC bioGUNE |
| contact email | mazkargorta@cicbiogune.es |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102069/ |
