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PXD079283-1

PXD079283 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome response of the diatom Phaeodactylum tricornutum to short term shifts in nitrogen source and availability
DescriptionThe photosynthetic model marine diatom Phaeodactylum tricornutum was cultured with varying nitrogen sources and availability to elicit shifts in the proteome. Cells were grown to mid-exponential phase on ammonium (880 uM), collected by centrifugation and resuspended in N-free media for 2 hrs, and spiked with nitrate (150 uM) for 90 min. Cells were again collected by centrifugation, washed in N-free media, and resuspended in experimental treatments with no nitrogen (N-) and a nitrogen source (300 uM) provided as ammonium, nitrite, or nitrate. Each experimental treatment was sampled after 15 min, 45 min, and 18 hr. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.
HostingRepositoryMassIVE
AnnounceDate2026-06-04
AnnouncementXMLSubmission_2026-06-04_17:28:38.300.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: Phaeodactylum tricornutum; NCBI TaxID: 2850;
ModificationListOxidation; Carbamidomethyl; iTRAQ8plex
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-06-04 16:43:38ID requested
12026-06-04 17:28:38announced
Publication List
no publication
Keyword List
submitter keyword: photosynthesis, diatom, DatasetType:Proteomics
Contact List
Andrew E. Allen
contact affiliationScripps Institution of Oceanography, University of California
contact emailaallen@jcvi.org
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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