PXD079272-1

PXD079272 is an original dataset announced via ProteomeXchange.

Title	Glycerol-Driven Energy and Proteostasis Underpin Antibiotic Tolerance in Escherichia coli
Description	Bacterial persisters are frequently described as metabolically dormant, yet the endogenous metabolic programs that sustain survival during prolonged nutrient limitation remain poorly understood. Here, using stationary-phase Escherichia coli as a model of antibiotic tolerance, we combine proteomics, genetics, metabolic phenotyping, and single-cell imaging to define a metabolic framework underlying persistence. Perturbation of tricarboxylic acid cycle function broadly reprogrammed stationary-phase physiology, suppressing lipid and glycerol metabolism, altering energy homeostasis and proteostasis, and reducing antibiotic tolerance. Systems-level analyses identified phospholipid-derived glycerol catabolism as a central metabolic node linking endogenous carbon recycling to persistence. Genetic disruption of glycerol utilization impaired proton motive force homeostasis, reduced formation of large polar protein aggregates, altered division-associated remodeling, and sensitized cells to antibiotic-induced lysis. Functional metabolic assays further revealed that persisters retain a selective capacity to utilize glycerol for rapid proton motive force restoration without growth resumption. Together, our findings support a model in which stationary-phase persisters are not metabolically inert but sustained through endogenous metabolic rewiring that coordinates energy maintenance, proteostasis, and antibiotic tolerance.
HostingRepository	PRIDE
AnnounceDate	2026-06-12
AnnouncementXML	Submission_2026-06-12_11:48:45.874.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Han Ngo
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-04 08:22:51	ID requested
⏵ 1	2026-06-12 11:48:46	announced

Dataset with its publication pending

submitter keyword: Persister

Escherichia coli

metabolism

TCA cycle

Protein aggregation

Large polar aggregates

Phospholipid recycling

Mehmet Orman
contact affiliation	Department of Biomedical Engineering, University of Wisconsin-Madison
contact email	maorman@wisc.edu
lab head
Han Ngo
contact affiliation	University of Wisconsin-Madison
contact email	hgngo@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079272
     1. Label: PRIDE project
     2. Name: Glycerol-Driven Energy and Proteostasis Underpin Antibiotic Tolerance in Escherichia coli