PXD079152-1

PXD079152 is an original dataset announced via ProteomeXchange.

Title	Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression
Description	Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) manipulates cellular processes through the translocation of effector molecules into the host cell cytosol. Using a recently established neonatal S. Typhimurium infection model, we provide functional insights into how Salmonella outer protein B (SopB) suppresses early mucosal tissue inflammation and prolongs host survival. Mechanistically, SopB prevents a disintegrin and metalloprotease 17 (ADAM17) activation, plasma membrane translocation and the release of membrane-bound TNFa from enterocytes and reduces epithelial secretion of IL-18 via mTOR controlled secretory autophagy. This abolishes the early epithelial transcriptional response and reduces immune cell recruitment and programmed cell death-mediated mucosal barrier disruption delaying disease progression. The immunosuppressive effect of SopB is independent of the C-terminally encoded phosphatidylinositol phosphatase and phosphotransferase activity but requires an intact N-terminal domain. Thus, here we demonstrate that SopB suppresses the early, post-transcriptional regulation of epithelial cytokine release in an inositol phosphatase independent manner likely promoting pathogen transmission.
HostingRepository	PRIDE
AnnounceDate	2026-07-07
AnnouncementXML	Submission_2026-07-06_22:35:21.337.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlene Birk
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Salmonella typhimurium (strain 14028s / SGSC 2262); NCBI TaxID: NEWT:588858;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-01 16:28:35	ID requested
⏵ 1	2026-07-06 22:35:22	announced

10.1038/s41467-026-74942-9;

Diab N, Yong CH, Stange EL, Birk MS, Schmitz MA, D, ü, sterh, ö, ft S, Pes J, Ferle KN, Karkossa I, Schubert K, Deiwick J, Vucur M, Luedde T, Torow N, Ludwig A, Dupont A, Selkrig J, von Bergen M, Hensel M, Zhang K, Hornef MW, Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression. Nat Commun, 17(1):(2026) [pubmed]

submitter keyword: LC-MSMS,Salmonella, SopB interactors, TMT

Prof. Dr. Mathias Walter Hornef
contact affiliation	Institute for Medical Microbiology Universitätsklinikum der RWTH Aachen, Germany
contact email	mhornef@ukaachen.de
lab head
Marlene Birk
contact affiliation	University Hospital Aachen
contact email	mbirk@ukaachen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079152
     1. Label: PRIDE project
     2. Name: Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression