PXD079145-1

PXD079145 is an original dataset announced via ProteomeXchange.

Title	Evaluation of FASP for mass spectrometry-based untargeted metabolomics analysis of urine samples
Description	Filter-Aided Sample Preparation (FASP) is a well-established method in proteomics, yet its potential for the parallel recovery of metabolites remains largely unexplored. Herein, we evaluate the performance of FASP as a straightforward workflow for the simultaneous isolation of protein and corresponding metabolite fractions from a single urine sample. The FASP-based LC-MS/MS approach for both proteomics and metabolomics analysis identified 3,163 non-redundant peptides corresponding to 957 unique protein groups. The metabolomic profile comparison of three urine fractions, specifically FASP-concentrated, FASP flow-through, and raw samples, resulted in the identification of 176 common metabolites. Next, as a proof-of-concept, the FASP protocol was applied to compare the metabolomic profiles of clinical urine samples from healthy individuals (n=13) and patients with Ta bladder cancer (n=12). The metabolomic modulation was consistent with previously reported findings, highlighting perturbations in phenylacetate, purine, and tryptophan metabolism, as reflected by changes in metabolites such as adenosine monophosphate (AMP), phenylacetic acid, glutamine, cytosine, and L-tryptophan. FASP protocol can be effectively adapted for the concurrent profiling of both proteomic and metabolomic fractions from urine samples. Thus, FASP-based workflow represents a viable alternative for single-step sample preparation, facilitating subsequent quantitative multi-omics data integration.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-06-01_12:13:15.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Muath Mousa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-01 12:04:03	ID requested
⏵ 1	2026-06-01 12:13:15	announced

Dataset with its publication pending

submitter keyword: FASP

Proteomics

Metabolomics

Multi-Omics

Bladder cancer

Urine

Sample preparation

Muath Khairi Mousa
contact affiliation	1) OMICS and Analytical Development Group, BIOSCOPE Research Group, LAQV REQUIMTE, Chemistry Department, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516, Caparica, Portugal
contact email	m.mousa@campus.fct.unl.pt
lab head
Muath Mousa
contact affiliation	BIOSCOPE Research Group, LAQV-REQUIMTE, Department of Chemistry, NOVA School of Science and Technology, NOVA University Lisbon 2829-516, Caparica, Portugal. Country: Portugal
contact email	m.mousa@campus.fct.unl.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD079145

PRIDE project URI

• PRIDE
  1. PXD079145
     1. Label: PRIDE project
     2. Name: Evaluation of FASP for mass spectrometry-based untargeted metabolomics analysis of urine samples