PXD079084-1

PXD079084 is an original dataset announced via ProteomeXchange.

Title	A RAF–SnRK2–PP2C functional module regulates cell survival in the chlorophyte Chlamydomonas reinhardtii
Description	The SNF1-related protein kinase 2 (SnRK2) pathway is a central regulator of abiotic stress signaling in land plants; however, its evolutionary origins and functional conservation across the green lineage remain poorly understood. Land plants evolved from streptophyte algae, whereas the chlorophyte algae, including the widely studied model Chlamydomonas reinhardtii, represent an older lineage that diverged from streptophytes over one billion years ago. Here, we identify and characterize a functional SnRK2 signaling module in Chlamydomonas reinhardtii. We show that the isoform SnRK2.7 is required for osmotic stress tolerance and general cellular viability, thus offering an alternative model for dissecting SnRK2 functions in algae. SnRK2.7 localizes to the contractile vacuole, an osmoregulatory organelle lost during streptophyte evolution, revealing a lineage-specific functional adaptation. Together with MAPKKK3, a B1/B3-RAF kinase, and the clade A protein phosphatase PP2C3, these components constitute a SnRK2 signaling module in Chlamydomonas reinhardtii. Our findings demonstrate the presence of a functional SnRK2 pathway in a chlorophyte alga, indicating that core components were established early in the green lineage. This work provides a foundation for comparative studies across green plants and underscores the need for broader taxonomic sampling to reconstruct the ancestral signaling networks underlying stress adaptation.
HostingRepository	PRIDE
AnnounceDate	2026-07-02
AnnouncementXML	Submission_2026-07-02_01:51:59.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	GKN JK
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: NEWT:3055;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-31 02:50:28	ID requested
⏵ 1	2026-07-02 01:52:00	announced

10.1111/NPH.71438;

submitter keyword: Chlamydomonas reinhardtii

xiaobo li
contact affiliation	Professor
contact email	lixiaobo@westlake.edu.cn
lab head
GKN JK
contact affiliation	Westlake University
contact email	guokangning@westlake.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD079084
     1. Label: PRIDE project
     2. Name: A RAF–SnRK2–PP2C functional module regulates cell survival in the chlorophyte Chlamydomonas reinhardtii