PXD078972-1

PXD078972 is an original dataset announced via ProteomeXchange.

Title	IL-33/ERK Signaling Promotes Neural Repair by Driving Functional Microglial Renewal
Description	Repair after central nervous system injury depends on remodeling of the immune microenvironment, in which microglial regeneration is critical. Here, we found that IL-33 promotes microglial regeneration through IL1RL1/ST2–ERK signaling and induces a reparative, inflammation-resolving phenotype characterized by high expression of TREM2, IGF1, TGF-β, and IL-10, and low expression of TNF-α, IL-1β, CD86, and FcγRIII. These newly generated microglia displayed enhanced phagocytic activity and lysosomal activation, reshaped cell–cell communication networks, and promoted hippocampal neurogenesis, synaptic repair, axonal regeneration, and cognitive recovery. IL1RL1 knockdown, ERK inhibition, or microglial depletion weakened the restorative effects of IL-33. Connectivity Map screening further identified curcumin as an IL-33-mimetic compound that promoted microglial renewal and phagocytosis both in vivo and in vitro. In a carbon monoxide poisoning model, CO exposure suppressed the expansion of proliferative microglial subsets and ERK phosphorylation, leading to impaired functional regeneration. Together, these findings suggest that the IL-33/IL1RL1–ERK axis promotes neural repair by inducing pro-efferocytic, low-inflammatory regenerative microglia, providing a potential immunomodulatory strategy for toxic brain injury.
HostingRepository	PRIDE
AnnounceDate	2026-08-03
AnnouncementXML	Submission_2026-08-03_00:49:18.798.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xudong Zhou
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-28 02:49:46	ID requested
⏵ 1	2026-08-03 00:49:19	announced

Dataset with its publication pending

submitter keyword: Microglia

IL-33

Autophagy

ERK

Neural repair

Carbon monoxide poisoning

Xudong Zhou
contact affiliation	Yantai Yuhuangding Hospital, Qingdao University.
contact email	Haipizxd@gmail.com
lab head
Xudong Zhou
contact affiliation	Yantai Yuhuangding Hospital, Qingdao University.
contact email	haipizxd@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078972

PRIDE project URI

• PRIDE
  1. PXD078972
     1. Label: PRIDE project
     2. Name: IL-33/ERK Signaling Promotes Neural Repair by Driving Functional Microglial Renewal