PXD078938-1

PXD078938 is an original dataset announced via ProteomeXchange.

Title	Agonist-Specific Modulation of the MyD88 Niche Governs Temporal Dynamics of Immune Responses
Description	Toll-like receptors (TLRs) are essential sentinels of innate immunity that detect pathogen-associated molecular patterns (PAMPs) and initiate downstream signaling cascades. Central to these pathways is the adaptor protein myeloid differentiation primary response 88 (MyD88), which functions as a pivotal hub coordinating early immune responses. Despite its importance, the temporal dynamics of MyD88-dependent signaling in response to distinct TLR agonists remain incompletely defined. In this study, we employed a systematic approach to dissect agonist-specific modulation of the MyD88 signaling niche in macrophages. Using a panel of canonical TLR ligands—lipopolysaccharide (LPS, TLR4), Pam3CysSerLys4 (P3C, TLR2/1), resiquimod (R848, TLR7/8), and polyinosinic-polycytidylic acid (poly(I:C), TLR3)—we demonstrate that different agonists drive unique temporal patterns of MyD88 engagement, resulting in distinct transcriptional programs and post-translational modifications. These findings reveal that MyD88 operates as a dynamic regulatory node whose functional output is shaped by the identity of the upstream agonist, thereby fine-tuning the magnitude and kinetics of innate immune activation.
HostingRepository	PRIDE
AnnounceDate	2026-08-13
AnnouncementXML	Submission_2026-08-13_04:06:36.186.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Nita-Lazar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-27 14:59:47	ID requested
⏵ 1	2026-08-13 04:06:36	announced

Dataset with its publication pending

submitter keyword: innate immunity, myddosome,Toll-like receptors, myeloid differentiation primary response gene 88, interactome.

ALEKSANDRA NITA-LAZAR
contact affiliation	NIH
contact email	NITALAZARAU@NIAID.NIH.GOV
lab head
Aleksandra Nita-Lazar
contact affiliation	NIAID
contact email	nitalazarau@niaid.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078938
     1. Label: PRIDE project
     2. Name: Agonist-Specific Modulation of the MyD88 Niche Governs Temporal Dynamics of Immune Responses