PXD078909 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Zebrafish quantitative proteomics |
| Description | Food allergies, including α-Gal syndrome (AGS), arise from complex interactions between environmental exposure and host intestinal responses that remain poorly defined. We used quantitative SWATH-MS proteomics to characterize zebrafish gut responses to Ixodes ricinus tick saliva or defined salivary protein fractions and subsequent meat consumption. Tick saliva induced coordinated downregulation of metabolic and redox pathways, indicating reduced intestinal functional capacity rather than a classical inflammatory response. The complete protein fraction generated a more fragmented network, combining metabolic suppression with proteostasis impairment and ectopic expression of vitellogenin-like proteins, suggestive of epithelial reprogramming. Individual fractions produced distinct but convergent phenotypes: F1 drove broad suppression of digestive enzymes and loss of enterocyte function; F4 disrupted proteostasis and epithelial integrity; and F5 induced widespread metabolic and mitochondrial dysregulation. Despite limited protein overlap, all fractions converged on metabolic, redox, proteostatic and epithelial collapse pathways, indicating that similar intestinal outcomes arise through distinct molecular routes. F1-associated induction of alpha-2-macroglobulin and vitellogenins suggest links between tick exposure, α-Gal responses and epithelial remodeling. These proteins may serve as biomarkers of AGS-related intestinal reprogramming, pending further validation. Overall, tick saliva acts as a modular system that reconfigures intestinal homeostasis and increases susceptibility to dietary challenges in an AGS-relevant contest |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-27 |
| AnnouncementXML | Submission_2026-05-27_10:24:55.015.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD078909 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Margarita Villar |
| SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-27 07:47:50 | ID requested | |
| ⏵ 1 | 2026-05-27 10:24:55 | announced | |
Publication List
Keyword List
| submitter keyword: α-Gal syndrome |
| epithelial reprogramming |
| intestinal proteomics |
| tick saliva |
| zebrafish model |
Contact List
| Margarita Villar |
|---|
| contact affiliation | SaBio (Health and Biotechnology), Instituto de Investigación en Recursos Cinegéticos (IREC, CSIC-UCLM-JCCM), University of Castilla-La Mancha, 13071, Ciudad Real, Spain. |
| contact email | margaritam.villar@uclm.es |
| lab head | |
| Margarita Villar |
|---|
| contact affiliation | University of Castilla- La Mancha |
| contact email | margaritam.villar@uclm.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD078909 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD078909
- Label: PRIDE project
- Name: Zebrafish quantitative proteomics
