PXD078908-1

PXD078908 is an original dataset announced via ProteomeXchange.

Title	A family of lanthipeptides with anti-phage function
Description	Bacteria produce natural products to adapt to their environments, with phage interactions as major ecological and evolutionary drivers. While some natural products protect bacteria from phages, the prevalence and diversity of chemical anti-phage defenses remain largely unexplored. Here, we identify a widespread family of lanthipeptide biosynthetic gene clusters (BGCs) in Actinobacteria, which we term lanthivirin BGCs, that confer anti-phage activity. Lanthivirin BGCs often colocalize with other defense systems in genomic defense islands. We demonstrate anti-phage activity both in a native Streptomyces context and through heterologous expression of six distinct lanthivirin systems. Mutational analyses of the biosynthetic machinery and core peptide show that lanthivirin maturation is required for anti-phage activity. Genetic and biochemical analyses during infection support a phage-protein–dependent inhibition of phage DNA replication, with no host toxicity. Our findings highlight the untapped potential of BGCs as sources of anti-phage natural products, offering a new avenue for discovering antiviral compounds.
HostingRepository	PRIDE
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-21_02:58:44.445.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chao DU
SpeciesList	scientific name: Streptomyces albidoflavus; NCBI TaxID: NEWT:1886;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-27 07:10:30	ID requested
⏵ 1	2026-07-21 02:58:44	announced

10.1016/j.chom.2026.06.017;

10.1016/J.CHOM.2026.06.017;

submitter keyword: biosynthetic gene clusters,lanthipeptide, Actinobacteria, anti-phage defense, natural products

Gilles van Wezel
contact affiliation	Institute of Biology Leiden, Leiden University
contact email	g.wezel@biology.leidenuniv.nl
lab head
Chao DU
contact affiliation	Leiden University
contact email	c.du@biology.leidenuniv.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078908
     1. Label: PRIDE project
     2. Name: A family of lanthipeptides with anti-phage function