PXD078872-1

PXD078872 is an original dataset announced via ProteomeXchange.

Title	POLR3A Crosslinking co-immunoprecipitate MS
Description	RNA polymerase III (Pol III) produces noncoding RNAs involved in diverse cellular activities, including translation (tRNA, 5S rRNA, 7SL RNA), RNA processing (U6 snRNA, RPPH1, RMRP), and transcription regulation (7SK snRNA). In this way, Pol III activity must be broadly coupled with cellular demands for protein accumulation and growth, increasing in response to nutrient availability and decreasing during differentiation and exit from proliferation. However, the currently established mechanisms of Pol III regulation remain relatively limited, due in part to the few Pol III-centered protein-protein interaction (PPI) studies performed to date. To address this gap, we first investigated PPIs shared by multiple Pol III subunits to understand the macromolecular interactome of Pol III, with special attention directed at potential regulatory candidates. Our proteomic survey uncovers interactions between Pol III and the NuRD (Nucleosome Remodeling and Deacetylase) complex. Taken further, we show that NuRD localizes to active Pol III-transcribed genes and that its recruitment is Pol III-dependent but nonrandom, with peak occupancy and regulatory hallmarks converging on tRNA gene clusters associated with notably high expression levels. Inhibiting NuRD-associated histone deacetylase function reduces Pol III transcription at these sites, suggesting NuRD restricts Pol III and thereby modulates the global dynamic range of Pol III-derived RNA species. These findings are congruent with the transcriptionally repressive nature of NuRD and bring-to-light a new regulatory mechanism that may couple signaling events and changes in metabolic needs with the dynamic availability of specific tRNA pools.
HostingRepository	PRIDE
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-02_23:58:53.196.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD078872
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ruiying Cheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	biotinylated residue; phosphorylated residue; acetylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-26 19:13:05	ID requested
⏵ 1	2026-09-02 23:58:53	announced

10.6019/PXD078872;

submitter keyword: POLR3A, Crosslinking co-immunoprecipitate

Kevin Van Bortle
contact affiliation	Department of Cell and Developmental Biology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA Cancer Center at Illinois, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA
contact email	kvbortle@illinois.edu
lab head
Ruiying Cheng
contact affiliation	University of Illinois Urbana-Champaign
contact email	rcheng14@illinois.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078872
     1. Label: PRIDE project
     2. Name: POLR3A Crosslinking co-immunoprecipitate MS