PXD078617-1

PXD078617 is an original dataset announced via ProteomeXchange.

Title	Sex-specific proteomic profiling identifies pregnancy zone protein as a complement-linked marker of adverse prognosis in esophageal adenocarcinoma
Description	Esophageal adenocarcinoma (EAC) exhibits marked male predominance with male-to-female ratios reaching 8.5:1, yet the molecular basis underlying this sex disparity remains poorly characterized. We analyzed 92 EAC specimens using mass spectrometry–based proteomics, comprising 47 female and 45 male tumors from treatment-naïve patients. Differential expression, pathway enrichment, immunohistochemical, and survival analyses were used to identify sex-associated proteomic features and prognostic signatures. Proteomic profiling revealed focal yet biologically meaningful sex differences in esophageal adenocarcinoma. After multiple testing correction, three proteins were differentially expressed: the autosomal protein pregnancy zone protein (PZP), enriched in female tumors, and the Y-chromosome–encoded proteins DDX3Y and RPS4Y1, which were overexpressed in male tumors. At nominal significance, proteins upregulated in female tumors showed marked enrichment of immune-related pathways. Proteins correlated with PZP expression formed a network dominated by complement cascade. Clinically, elevated PZP expression was associated with significantly poorer overall survival, most pronounced in male patients and independent of sex-specific survival differences. Proteome-wide survival analyses further demonstrated distinct sex-specific prognostic landscapes. Our study provides the first comprehensive characterization of sex-associated proteomic differences in EAC. Sex shapes immune-related pathways, prognostic proteomic signatures, and survival associations. PZP emerges as a sex-differential, complement-associated protein with adverse prognostic significance, highlighting sex as a biologically and clinically relevant variable in EAC
HostingRepository	PRIDE
AnnounceDate	2026-06-25
AnnouncementXML	Submission_2026-06-25_01:25:52.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Facility
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-20 07:37:44	ID requested
⏵ 1	2026-06-25 01:25:53	announced

Dataset with its publication pending

submitter keyword: Esophageal adenocarcinoma

Sex differences

Proteomics

Pregnancy zone protein

Prof. Dr. A. Quaas
contact affiliation	Institut für Pathologie Uniklinik Köln, Kerpener Straße 62, 50937 Köln
contact email	alexander.quaas@uk-koeln.de
lab head
Proteomics Facility
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078617
     1. Label: PRIDE project
     2. Name: Sex-specific proteomic profiling identifies pregnancy zone protein as a complement-linked marker of adverse prognosis in esophageal adenocarcinoma