⮝ Full datasets listing
PXD078604-1
PXD078604 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Androgen Rapidly Activates Phosphorylation Signaling Pathways in Sertoli Cells |
| Description | This study comprehensively analyzed androgen-activated phosphorylation signaling pathways in testicular Sertoli cells. Primary cultured mouse Sertoli cells were treated with testosterone for varying durations. Using Tandem Mass Tag (TMT)-labeled phosphoproteomics technology combined with Mfuzz clustering and functional enrichment analysis, we identified key phosphorylation signaling pathways. Mass spectrometry revealed 13,015 quantifiable phosphorylation sites, and Mfuzz clustering of expression pattern categorized 330 proteins into 6 clusters. Functional enrichment analysis highlighted key pathways, including Rap1 and MAPK kinases. Inhibitor experiments confirmed that the disruption of phosphorylation signaling impaired the integrity of the blood-testis barrier. Collectively, these findings demonstrate that androgens rapidly activate the protein phosphorylation signaling network in Sertoli cells, regulating critical processes such as blood-testis barrier formation, thereby providing new insights into male fertility and potential therapeutic strategies for spermatogenic disorders. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-20 |
| AnnouncementXML | Submission_2026-05-20_02:16:40.521.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qiong Deng |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | phosphorylation to amine thiol |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-20 02:14:06 | ID requested | |
| ⏵ 1 | 2026-05-20 02:16:41 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Androgen, Sertoli cells, Phosphorylation |
Contact List
| Qiong Deng | |
|---|---|
| contact affiliation | Southern University of Science and Technology |
| contact email | dengqiong1987@smu.edu.cn |
| lab head | |
| Qiong Deng | |
| contact affiliation | Southern University of Science and Technology |
| contact email | dengqiong1987@smu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
