PXD078529-1

PXD078529 is an original dataset announced via ProteomeXchange.

Title	FAHFAs are detected in postprandial chylomicron and VLDL fractions and can be released from TG estolides by LPL in vitro
Description	Fatty acid esters of hydroxy fatty acids (FAHFAs) are bioactive lipids with anti-diabetic and anti-inflammatory effects, but the mechanisms responsible for their transport in blood are not well understood. In this study, we investigated potential serum carriers of FAHFAs. Microscale thermophoresis showed that binding of 9-PAHSA to serum albumin did not support albumin as the dominant carrier under the conditions tested. We therefore turned our attention to lipoproteins and extracellular vesicles, which were isolated from murine serum by density-based ultracentrifugation and characterized by proteomics and lipidomics. Lipidomic analysis revealed that chylomicrons, very-low-density lipoproteins (VLDL), and low-density lipoproteins (LDL) contain both FAHFAs and their storage form, triacylglycerol estolides (TG-EST). In contrast, no FAHFA-containing lipids were detected in serum extracellular vesicles. As a validation case study, we confirmed the presence of TG-EST in postprandial chylomicrons from human volunteers. Functional assays further showed that lipoprotein lipase (LPL), the enzyme that hydrolyzes triacylglycerols during lipoprotein metabolism, releases FAHFAs from TG-EST. LPL exhibited positional selectivity, releasing distinct PAHSA regioisomers with different efficiencies. Together, these results support the interpretation that FAHFA-related lipids are associated with chylomicron, VLDL, and LDL fractions in the postprandial setting and establish LPL-mediated lipolysis as a potential mechanism for the release and distribution of these bioactive lipids in the circulation.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:28:34.083.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marek Vrbacky
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-18 22:53:03	ID requested
⏵ 1	2026-06-28 16:28:34	announced

10.1016/j.jlr.2026.101059;

Milonaityte D, Brejchova K, Srp J, Kudova E, Chodounska H, Balas L, Durand T, Abumrad NA, Kuda O, FAHFAs are detected in postprandial chylomicron and VLDL fractions and can be released from TG estolides by LPL in vitro. J Lipid Res, 67(6):101059(2026) [pubmed]

submitter keyword: Lipoproteins, Lipase,Fatty acid Transport, VLDL, Lipidomics

Ondrej Kuda
contact affiliation	Laboratory of Metabolism of Bioactive Lipids, Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic
contact email	ondrej.kuda@fgu.cas.cz
lab head
Marek Vrbacky
contact affiliation	Czech Academy of Sciences
contact email	proteom.krc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD078529
     1. Label: PRIDE project
     2. Name: FAHFAs are detected in postprandial chylomicron and VLDL fractions and can be released from TG estolides by LPL in vitro