PXD078528 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cancer cell-intrinsic rewiring of the colorectal cancer secretome |
| Description | Secreted factors are essential mediators of cellular communication that are exploited by tumors to control their environment and evade immune attack. However, how cancer cells rewire their secretome is largely unclear due to challenges in reliably profiling cancer cell-specific secreted factors. Here, we apply a click chemistry-based approach to selectively profile the secretome of patient-derived colorectal cancer (CRC) and healthy colon organoids with high resolution. We observe a major rewiring of the secretome in the transition to malignancy, with clear separation between microsatellite stable (MSS) and instable cancers. We then leverage this secretomics dataset to identify secreted factors that mark immune-excluded cancers, revealing NOTUM as secreted protein associating with decreased T cell infiltration across four independent datasets. Overall, our study provides an in-depth characterization of the cancer cell-intrinsic secretome of MSS-CRC and nominates NOTUM as a secreted factor marking immune excluded colorectal cancers. This provides a scalable framework to interrogate the cancer cell-intrinsic secretome and link secreted factors to relevant tumor phenotypes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-28 |
| AnnouncementXML | Submission_2026-08-28_02:04:35.671.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Onno Bleijerveld |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-18 22:06:02 | ID requested | |
| ⏵ 1 | 2026-08-28 02:04:36 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: organoids, NOTUM, proteomics,colorectal cancer, secretome, throncat |
Contact List
| Onno Bleijerveld |
|---|
| contact affiliation | Netherlands Cancer Institute Proteomics Facility |
| contact email | o.bleijerveld@nki.nl |
| lab head | |
| Onno Bleijerveld |
|---|
| contact affiliation | The Netherlands Cancer Institute |
| contact email | o.bleijerveld@nki.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078528 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD078528
- Label: PRIDE project
- Name: Cancer cell-intrinsic rewiring of the colorectal cancer secretome
