PXD078510 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | UFL1-Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer Progression |
| Description | Metabolic reprogramming toward enhanced aerobic glycolysis is a defining feature of cancer, yet how the contribution of ubiquitin-like modifications to this process remains poorly understood. Here, we identify the UFMylation E3 ligase UFL1 as a critical suppressor of glycolytic metabolism and colorectal cancer (CRC) progression. Mechanistically, UFL1 directly interacts with the glycolytic enzyme enolase 1 (ENO1) and catalyzes its UFMylation at lysine residues K285 and K420. This modification disrupts ENO1 dimerization, attenuates its enzymatic activity, and consequently suppresses glycolytic flux. Functionally, UFL1-mediated UFMylation restrains tumor growth in vitro and in vivo. Notably, pharmacological enhancement of the UFL1-ENO1 interaction using the FDA-approved antibiotic torezolid significantly potentiates the antitumor efficacy of 5-fluorouracil (5-FU) across multiple preclinical models, including CRC patient-derived organoids and xenografts, without detectable toxicity. Collectively, these findings establish ENO1 as a direct UFMylation substrate, reveal UFMylation as a previously unrecognized regulator of cancer metabolic reprogramming, and highlight the UFL1-ENO1 axis as a promising therapeutic target in colorectal cancer. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-02 |
| AnnouncementXML | Submission_2026-08-02_05:16:20.600.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiuqing Ma |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Ascend |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-18 11:44:21 | ID requested | |
| ⏵ 1 | 2026-08-02 05:16:21 | announced | |
Publication List
Keyword List
| submitter keyword: UFMylation |
| UFL1 |
| ENO1 |
| Colorectal cancer |
| Aerobic glycolysis; |
Contact List
| Tongzheng Liu |
|---|
| contact affiliation | State Key Laboratory of Bioactive Molecules and Druggability Assessment, Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative Drugs, School of Pharmacy, Jinan University, Guangzhou, 510632, China |
| contact email | liutongzheng@jnu.edu.cn |
| lab head | |
| Xiuqing Ma |
|---|
| contact affiliation | Jinan University |
| contact email | maxiuqing@stu2024.jnu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078510 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD078510
- Label: PRIDE project
- Name: UFL1-Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer Progression
