⮝ Full datasets listing
PXD078443-1
PXD078443 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | FLAG-IP–MS profiling of SPINDOC interactome using a HeLa–Tet-On–FLAG-SPINDOC-GFP cell line |
| Description | HeLa–Tet-On–FLAG-SPINDOC-GFP cells were established for inducible expression and affinity purification. FLAG-SPINDOC-GFP expression was first induced by doxycycline treatment. Subsequently, cells were either left unsynchronized or arrested at the G2/M phase by nocodazole treatment. Efficient expression and enrichment of FLAG-SPINDOC-GFP were confirmed by FLAG co-immunoprecipitation. Immunoprecipitates were then subjected to mass spectrometry analysis to identify SPINDOC-associated proteins, particularly under mitotic conditions. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-15 |
| AnnouncementXML | Submission_2026-05-17_23:48:45.088.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zixu Zhang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-17 23:48:15 | ID requested | |
| ⏵ 1 | 2026-05-17 23:48:45 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SPINDOC, doxycycline-inducible expression, FLAG-IP-MS, mitosis, HeLa cells |
Contact List
| Guohong Li | |
|---|---|
| contact affiliation | Wuhan University |
| contact email | liguohong@whu.edu.cn |
| lab head | |
| Zixu Zhang | |
| contact affiliation | Institute of Biophysics, Chinese Academy of Sciences |
| contact email | zzx772779243@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
