PXD078419-1

PXD078419 is an original dataset announced via ProteomeXchange.

Title	Oligodendroglioma cells modulate tumour growth via extracellular vesicle biogenesis
Description	Oligodendroglioma driver mutations are known, yet signature slow growth rates of these glial tumours are poorly understood. Here we show using patient biopsies that neoplastic and non-neoplastic cells in the tumour mass display elevated ERK signaling, proliferate ectopically, and undergo cell death. Oligodendroglioma cell cross-talk with the tumour microenvironment is mediated partly by extracellular vesicles, which inhibit astrocyte expansion in vitro. Elevated extracellular vesicle biogenesis gene (SMPD3,TSG101) expression positively correlates with longer patient survival. Accordingly, SMPD3 and TSG101 knock-down enhances oligodendroglioma cell expansion whereas overexpression slows cell growth. SMPD3 knock-down also enhances oligodendroglioma growth in cortical xenografts and in human cerebral organoids. Shared signature features of ODG cell line-derived vesicular proteomes include ribosomal biogenesis proteins, whereas the soluble secretome is growth factor-rich, the pro-proliferative effects of which are blocked by foretinib. Oligodendroglioma cell growth is thus controlled by the combined effects of cytostatic/cytotoxic extracellular vesicles and soluble pro-proliferative growth factors.
HostingRepository	PRIDE
AnnounceDate	2026-07-01
AnnouncementXML	Submission_2026-07-01_08:55:46.963.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD078419
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Olender
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-15 14:45:05	ID requested
⏵ 1	2026-07-01 08:55:47	announced

10.6019/PXD078419;

10.1038/s42003-026-10556-7;

Saleh F, Balakrishnan A, Adnani L, Ahmad ST, Chinchalongporn V, El-Sehemy A, Olender T, Chen MJ, Kshirsagar A, Kennedy A, Kulathunga N, Prokopchuk O, Vasan L, Touahri Y, Islam R, Sujanthan S, Zinyk D, Comanita LC, Kan B, Fleming T, Michael IP, Morshead CM, Leong HS, Okawa S, Brand M, Wallace VA, Chan JA, Schuurmans C, SMPD3 suppresses oligodendroglioma growth via dual autocrine-paracrine roles. Commun Biol, ():(2026) [pubmed]

submitter keyword: Human, BT88, Oligodendroglioma, BT54, Extracellular Vesicles, BT054, BT088

Carol Schuurmans
contact affiliation	Sunnybrook Research Institute, Toronto, ON, Canada / Department of Biochemistry, University of Toronto, Toronto, ON, Canada / Department of Biochemistry and Molecular Biology, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada / Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada
contact email	carol.schuurmans@sunnybrook.ca
lab head
Thomas Olender
contact affiliation	Ottawa Hospital Research Institute and The University of Ottawa (Ottawa, Ontario Canada).
contact email	thomas.c.olender@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD078419

PRIDE project URI

• PRIDE
  1. PXD078419
     1. Label: PRIDE project
     2. Name: Oligodendroglioma cells modulate tumour growth via extracellular vesicle biogenesis