PXD078353-1

PXD078353 is an original dataset announced via ProteomeXchange.

Title	Regulation of the histone H3K36 methyltransferase Set2 by the histone chaperone Spt6 – HDX-MS dataset
Description	Histone H3 lysine 36 methylation is a conserved histone modification that is critical for maintaining eukaryotic transcriptional fidelity and genomic stability. In yeast, this modification is catalyzed by Set2, an ortholog of the mammalian H3K36 methyltransferase SETD2. Previous genetic, biochemical, and structural studies showed that Set2 activity is repressed by a Set2 autoinhibitory domain (AID) and that activation requires the direct binding of the histone chaperone Spt6. To study the role of Spt6 and Set2 autoinhibition in vivo, we have isolated and analyzed multiple classes of Spt6 and Set2 mutants. Our results suggest an autoinhibited form of Set2 in which the catalytic domain is bound by the AID. In strong agreement with our genetic results, biophysical experiments demonstrate that the Set2 catalytic domain and Set2 AID physically interact, and that the autoinhibition mutants abolish this interaction. The hydrogen deuterium exchange was used to study effect of mutations on the protein structure.
HostingRepository	PRIDE
AnnounceDate	2026-08-31
AnnouncementXML	Submission_2026-08-31_01:31:39.017.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tereza Nesporova
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	SELECT SERIES Cyclic IMS

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-14 05:34:13	ID requested
⏵ 1	2026-08-31 01:31:39	announced

Dataset with its publication pending

submitter keyword: structural proteomic,histone H3K36 methyltransferase Set2, HDX-MS

Frantisek Filandr
contact affiliation	Institute of Organic Chemistry and Biochemistry of the CAS, Prague 16000, Czech Republic
contact email	frantisek.filandr@uochb.cas.cz
lab head
Tereza Nesporova
contact affiliation	Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic
contact email	tereza.nesporova@uochb.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD078353

PRIDE project URI

• PRIDE
  1. PXD078353
     1. Label: PRIDE project
     2. Name: Regulation of the histone H3K36 methyltransferase Set2 by the histone chaperone Spt6 – HDX-MS dataset