PXD078350 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: HYE Quantification Data Sets for PAMAF Mode |
| Description | Here we present a parallel accumulation-mobility aligned fragmentation (PAMAF) mode that expands the capabilities of the MOBILion Systems MOBIE® high-resolution ion mobility (HRIM) system for mass spectrometry-based proteomics. The MOBIE system leverages structures for lossless ion manipulation (SLIM) to achieve HRIM separations within a compact footprint. Along with the HRIM separations, which increase the number of features identified per cycle, PAMAF enables alignment of fragment ions with their precursor ions without quadrupole isolation prior to fragmentation, avoiding the ion losses up to 99%. With PAMAF mode, ions can be accumulated in parallel to the ion mobility (IM) separation, achieving close to 100% ion utilization efficiency. Here, we demonstrate deep proteome coverage and robust LFQ accuracy and precision using PAMAF on a HRIM-QTOF platform. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-11 |
| AnnouncementXML | Submission_2026-07-11_12:47:18.465.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leonard Rorrer |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Mobilion MBI format; Agilent instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-14 04:55:17 | ID requested | |
| ⏵ 1 | 2026-07-11 12:47:19 | announced | |
Publication List
| Rorrer LC, Deng L, Royer L, Uribe I, Orsburn BC, Bernhardt O, Gandhi T, Reiter L, DeBord D, A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High-Performance Proteomics Analysis. Mol Cell Proteomics, 25(7):101608(2026) [pubmed] |
| 10.1016/j.mcpro.2026.101608; |
Keyword List
| submitter keyword: PAMAF, Ion Mobility, QTOF, SLIM,HYE, E. Coli, Yeast, HeLa, HRIM, Bottom-Up Proteomics |
Contact List
| Daniel DeBord |
|---|
| contact affiliation | MOBILion Systems, Inc |
| contact email | daniel.debord@mobilionsystems.com |
| lab head | |
| Leonard Rorrer |
|---|
| contact affiliation | MOBILion Systems, Inc. |
| contact email | leonard.rorrer@mobilionsystems.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD078350 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD078350
- Label: PRIDE project
- Name: A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High Performance Proteomics Analysis: HYE Quantification Data Sets for PAMAF Mode
