⮝ Full datasets listing
PXD078149-1
PXD078149 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Visual exoproteomics of Clostridium thermocellum during anaerobic biomass-degradation identifies functional spirosomes |
| Description | This project utilizes a visual proteomics approach to characterize the exoproteome of the anaerobic, cellulose-degrading bacterium Clostridium thermocellum during biomass degradation. By integrating mass spectrometry with high-resolution cryo-electron microscopy (cryo-EM), we interrogated the composition of large exoproteome assemblies and extracellular machinery. Mass spectrometry analysis confirmed a broad exoproteome composition, notably identifying key cellulose-degrading components such as CelA and CipA. Further proteomic investigation of high-abundance protein filaments and vesicular structures, combined with de novo modeling, identified the presence of functional aldehyde-alcohol dehydrogenase (AdhE) spirosomes. This dataset contains the mass spectrometry evidence used to support protein identification and structural characterization of these extracellular complexes, providing insight into the functional exoproteome landscape of C. thermocellum. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-21 |
| AnnouncementXML | Submission_2026-05-21_17:17:25.251.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Matt Kensil |
| SpeciesList | scientific name: Clostridium thermocellum DSM 1313; NCBI TaxID: 637887; scientific name: Hungateiclostridium thermocellum; NCBI TaxID: 1515; |
| ModificationList | Oxidation; Acetyl; Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-08 00:13:54 | ID requested | |
| ⏵ 1 | 2026-05-21 17:17:25 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: cryoEM, biomass degradation, visual proteomics, cellulose, cellulosome, spirosome, DatasetType:Proteomics |
Contact List
| Joseph A. Loo | |
|---|---|
| contact affiliation | University of California, Los Angeles |
| contact email | jloo@chem.ucla.edu |
| lab head | |
| Jose Rodriguez | |
| contact affiliation | University of California, Los Angeles |
| contact email | jrodriguez@mbi.ucla.edu |
| lab head | |
| Matt Kensil | |
| contact affiliation | UCLA |
| contact email | mkensil@g.ucla.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101762/ |
