PXD078134-1

PXD078134 is an original dataset announced via ProteomeXchange.

Title	Identification of DELLA proximitome by TurboID labeling
Description	DELLA proteins act as transcription regulators that repress gibberellin (GA) responses and are best known for controlling the semi-dwarf crop varieties that drove the Green Revolution. DELLAs regulate gene expression primarily through protein–protein interactions with transcription factors and chromatin regulators. Recent work further indicates that DELLA activity involves formation of transcription factor–DELLA–histone H2A complexes at target loci and recruitment of the Mediator coactivator complex via the tail subunit MED15. Although DELLAs are known phosphoproteins, the kinases responsible and the functional significance of DELLA phosphorylation—particularly independent of GA‑induced proteolysis—remain unknown. To identify kinase(s) that associate with the Arabidopsis DELLA protein RGA in planta, we performed TurboID (TbID) proximity labeling to capture target proteins such as protein kinases that may only transiently interact with RGA in planta. RGA proximitome includes the CDK8 kinase module of the Mediator complex and a broad set of DELLA-associated transcription regulators.
HostingRepository	PRIDE
AnnounceDate	2026-07-28
AnnouncementXML	Submission_2026-07-28_10:34:18.206.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-07 13:26:55	ID requested
⏵ 1	2026-07-28 10:34:18	announced

Dataset with its publication pending

submitter keyword: DELLA proximitome, TurboID,DELLA proteins, proximity labeling, LC-MS/MS

Shouling Xu
contact affiliation	Department of Plant Biology, Carnegie Institution for Science, Stanford, California, USA
contact email	sxu@carnegiescience.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD078134

PRIDE project URI

• PRIDE
  1. PXD078134
     1. Label: PRIDE project
     2. Name: Identification of DELLA proximitome by TurboID labeling