PXD078105 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Functional and Structural Characterization of the Erythrina fusca Seed Lectin EFusL: A GalNAc-Binding Lectin with Specificity for Tumor-Associated O-Glycans |
| Description | The seed lectin from Erythrina fusca (EFusL) was purified and characterized to provide an integrated biochemical, structural, and biological profile of this less-described member of the genus Erythrina. EFusL was isolated by affinity chromatography on agarose-lactose and evaluated through hemagglutination assays, physicochemical stability tests, sequence determination, and toxicity assays using Artemia salina nauplii and human cell lines. The purified protein was identified as a 29–31 kDa glycoprotein containing approximately 4.3% neutral carbohydrates and showed specificity for galactosides, with strong inhibition by α-lactose, D-galactose, and GalNAc. EFusL displayed maximal hemagglutinating activity at pH 8.0, retained stability up to 40 °C, and required Ca²⁺ and Mn²⁺ ions for activity recovery. Its 249-amino acid sequence showed high similarity to other Erythrina lectins and conserved key carbohydrate-recognition residues. No toxicity was observed in the tested biological models. These findings indicate that EFusL preserves the canonical legume lectin features and support its potential use as a biochemical and biotechnological tool. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-05 |
| AnnouncementXML | Submission_2026-09-05_07:18:22.734.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD078105 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Messias Oliveira |
| SpeciesList | scientific name: Erythrina fusca; NCBI TaxID: NEWT:556509; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q-Tof Premier |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-07 02:55:51 | ID requested | |
| ⏵ 1 | 2026-09-05 07:18:23 | announced | |
| 2 | 2026-09-05 07:45:38 | announced | 2026-09-05: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: Leguminosae, β-sandwich, Glycoprotein, Phaseoleae, Hemagglutinin |
Contact List
| Kyria Santiago do Nascimento |
|---|
| contact affiliation | Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab Universidade Federal do Ceará - Campus do Pici Departamento de Bioquímica e Biologia Molecular - Bloco 907 Lab 1075 Av. Humberto Monte s/n | Pici | Fortaleza-CE | CEP: 60.440-970 |
| contact email | kyriasantiago@gmail.com |
| lab head | |
| Messias Oliveira |
|---|
| contact affiliation | Universidade Federal do Ceará |
| contact email | messiassigma@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD078105 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD078105
- Label: PRIDE project
- Name: Functional and Structural Characterization of the Erythrina fusca Seed Lectin EFusL: A GalNAc-Binding Lectin with Specificity for Tumor-Associated O-Glycans
