⮝ Full datasets listing
PXD078053-1
PXD078053 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Divalent Aptamer-mediated Clustering for Extracellular Vesicle Separation |
| Description | Extracellular vesicles (EVs) are important mediators of intercellular communication and promising sources of diagnostic and therapeutic biomarkers, yet effective EV isolation remains challenging due to trade-offs among yield, purity, and adaptability across biofluids. Here, we introduce divalent aptamer-mediated clustering (DAC), a streamlined affinity-based EV isolation strategy that induces controllable vesicle clustering and enables recovery by standard filtration. By exploiting multivalent aptamer binding to EV surface markers, DAC converts nanoscale vesicles into micron-scale clusters while preserving EV integrity and biological activity. We demonstrate robust EV isolation from plasma, urine, and cell culture media, and benchmark DAC against ultracentrifugation, density gradient ultracentrifugation, and size-exclusion chromatography. DAC achieves comparable or improved EV yield and purity with reduced processing time, cost, and operational complexity. Proteomic and metabolomic analyses show that DAC isolates affinity-defined EV subpopulations with cargo profiles distinct from those obtained using conventional methods. Moreover, DAC is readily adapted to alternative EV targets, exemplified by enrichment of EpCAM-positive EVs. Together, DAC provides a versatile and accessible platform for studying EV heterogeneity, function, and molecular composition. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-03 |
| AnnouncementXML | Submission_2026-05-06_01:54:05.355.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiaoling Liu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-06 01:53:44 | ID requested | |
| ⏵ 1 | 2026-05-06 01:54:06 | announced | |
| 2 | 2026-06-08 02:26:58 | announced | 2026-06-08: Add info |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Human,Plasma,extracellular vesicles,LC-MSMS |
Contact List
| Yaxuan Liang | |
|---|---|
| contact affiliation | Department of Biology, Faculty of Arts and Sciences, Beijing Normal University at Zhuhai 519087, China |
| contact email | yaxuan.liang9@gmail.com |
| lab head | |
| Xiaoling Liu | |
| contact affiliation | Beijing Normal University |
| contact email | 1451233915@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
