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PXD078046-2
PXD078046 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification and Analysis of Fha-Interacting Proteins in Vibrio cholerae |
| Description | This study aimed to identify proteins interacting with Fha in Vibrio cholerae. A Strep-tagged Fha fusion protein (Fha-Strep) was expressed in Vibrio cholerae, and associated protein complexes were enriched using Strep-Tactin affinity purification following cell lysis. As a negative control, an sfGFP-Strep fusion protein was expressed and processed in parallel. The enriched protein samples were subjected to tryptic digestion and analyzed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) using a timsTOF Pro mass spectrometer operated in dia-PASEF mode. This approach enabled comprehensive identification and quantification of Fha-associated proteins. Three biological replicates were included for each group to ensure reproducibility. The resulting dataset provides a resource for understanding the interaction network of Fha and its potential role in the Type VI Secretion System (T6SS) in Vibrio cholerae. |
| HostingRepository | iProX |
| AnnounceDate | 2026-05-01 |
| AnnouncementXML | Submission_2026-05-28_00:18:42.361.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tongtong Pei |
| SpeciesList | scientific name: Vibrio cholerae O1 biovar El Tor str. N16961; NCBI TaxID: 243277; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-05-06 01:36:13 | ID requested | |
| 1 | 2026-05-06 01:39:55 | announced | |
| ⏵ 2 | 2026-05-28 00:18:43 | announced | 2026-05-28: Add info |
Publication List
| Pei TT, Chen QY, Wang XY, Ma A, Liang JX, Ye ZY, Liu YZ, Su JT, Liang X, An Y, Zhu J, Dong T, Gene-in-gene coding generates dual-isoform Fha condensates to control type VI secretion system assembly. Nat Commun, ():(2026) [pubmed] |
Keyword List
| submitter keyword: Fha, T6SS, Vibrio cholerae |
Contact List
| Tao Dong | |
|---|---|
| contact affiliation | Southern University of Science and Technology |
| contact email | dongt@sustech.edu.cn |
| lab head | |
| Tongtong Pei | |
| contact affiliation | Southern University of Science and Technology |
| contact email | peitt@sustech.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
