PXD078003-1

PXD078003 is an original dataset announced via ProteomeXchange.

Title	Effect of Magnesium ions on MEC performance and changes in proteome landscape in T. acetexigens
Description	Microbial electrochemical systems (MECs) exploit the ability of electrogenic bacteria to transfer electrons to solid electrodes, thereby generating electricity or driving chemical synthesis. Model organisms such as Geobacter sulfurreducens and Shewanella oneidensis demonstrate how extracellular electron transfer (EET) can be harnessed in microbial fuel cells and electrolysers. While these systems show great promise, the performance of these devices depends on numerous factors, including pH, ionic strength and nutrient availability. Among those, magnesium has emerged as an especially important micronutrient because of its integral role in microbial physiology and its potential to influence electron transfer rates.This study examines how magnesium availability affects T. acetexigens performance. T. acetexigens is notable for its ability to produce current densities exceeding 9 A m-2 and possesses multiple co-existing EET pathways including large multi-heme cytochromes with up to 86 heme-binding motifs. Previously, we observed that the requirements for magnesium in growth media are significantly higher for T. acetexigens, compared to other EET bacteria, such as G. sulfurreducens. DSMZ (German Collection of Microorganisms) medium 148 for T. acetexigens specifies 1.6 mM MgSO4, whereas medium 826 for G. sulfurreducens contains only 0.12 mM. This represents a more than 13-fold difference in magnesium requirements. This observation prompted us to investigate how magnesium affects EET mechanisms, survivability and biofilm development along with associated changes on proteome level in T. acetexigens.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_08:50:12.590.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maksim Sysoev
SpeciesList	scientific name: Desulfuromonas acetexigens; NCBI TaxID: NEWT:38815;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-05 05:44:33	ID requested
⏵ 1	2026-05-13 08:50:13	announced

Dataset with its publication pending

submitter keyword: proteomics, DIA,MEC, EET, T. acetexigens

Maksim Sysoev
contact affiliation	King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science and Engineering Division (BESE)
contact email	maksim.sysoev@kaust.edu.sa
lab head
Maksim Sysoev
contact affiliation	King Abdullah University of Science and Technology
contact email	maksim.sysoev@kaust.edu.sa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD078003

PRIDE project URI

• PRIDE
  1. PXD078003
     1. Label: PRIDE project
     2. Name: Effect of Magnesium ions on MEC performance and changes in proteome landscape in T. acetexigens