PXD077990 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The deubiquitinase USP4 stabilizes the SCFFBXO24 ubiquitin E3 ligase to regulate immunity in experimental pneumonia |
| Description | The ubiquitin E3 ligase SCFFBXO24 targets key proteins for degradation that regulate important biological processes, but the mechanisms controlling cellular concentrations of its receptor subunit, F-box only protein 24 (FBXO24), remain unknown. Here, Haemophilus influenzae type B (HiB), an important cause of pneumonia, protects FBXO24 from lysosomal degradation in THP-1 macrophage cells by reducing its ubiquitylation levels. We identified that the ubiquitin-specific peptidase 4 (USP4) mediates FBXO24 deubiquitylation and stabilization in response to HiB infection in a TLR4 receptor-dependent manner. In experimental HiB pneumonia, either nanoparticle delivery of encapsulated USP4 siRNA or targeted genetic disruption of Fbxo24 in mice led to increased alveolar mononuclear cells coupled with reduced bacterial loads, attenuated pulmonary edema and decreased lung injury severity compared to control mice. Collectively, these findings are the first demonstrating ability of a bacterial pathogen to exploit a deubiquitylation mechanism to preserve cellular levels of an E3 ligase component, thereby impairing innate host defense responses. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-14 |
| AnnouncementXML | Submission_2026-08-14_08:06:18.441.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD077990 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | ilknur Yurtsever |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | ubiquitination signature dipeptidyl lysine |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-04 13:29:03 | ID requested | |
| ⏵ 1 | 2026-08-14 08:06:18 | announced | |
Publication List
Keyword List
| submitter keyword: USP4, deubiquitylation, Haemophilus influenzae,FBXO24 |
Contact List
| Rama K. Mallampalli |
|---|
| contact affiliation | Department of Internal Medicine, Division of Pulmonary, Critical Care and Sleep Medicine, Davis Heart and Lung Institute, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH, USA |
| contact email | Rama.Mallampalli2@osumc.edu |
| lab head | |
| ilknur Yurtsever |
|---|
| contact affiliation | Pulmonary Crıtıcal Care, Sleep, Wexner Medical Center, Ohio State University |
| contact email | yurt02@osumc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077990
- Label: PRIDE project
- Name: The deubiquitinase USP4 stabilizes the SCFFBXO24 ubiquitin E3 ligase to regulate immunity in experimental pneumonia
