PXD077957-1

PXD077957 is an original dataset announced via ProteomeXchange.

Title	Dual Preconditioned Mesenchymal Stem Cells-Derived Secretome Augment Immunomodulation and Drive Metabolic Reprogramming in Acute Graft-versus-Host Disease
Description	The immune plasticity of the host critically influences disease progression and therapeutic outcomes. In acute graft-versus-host disease (aGVHD), conventional conditioning regimens often induce toxicity, underscoring the need for effective adjunctive strategies to restore immune balance. This study evaluates the immunomodulatory and regenerative potential of secretome derived from naive and preconditioned human mesenchymal stem cells (MSCs) in aGVHD. Human MSCs from bone marrow and Wharton’s Jelly were preconditioned under hypoxia (1% O₂), apoptosis (1 µM staurosporine, 24 h), or both (dual preconditioning), and their secretome was assessed for immunoregulatory and antioxidant effects using T-cell proliferation assays, Treg induction, and M1-to-M2 macrophage polarization. Cellular bioenergetics and mitochondrial function were analyzed to explore metabolic reprogramming. Mechanistic insights were obtained through LC-MS/MS-based proteomic profiling of CCM co-cultured with aGVHD patient-derived activated PBMNCs, and in vivo efficacy was validated in a chemotherapy-induced aGVHD murine model. Dual preconditioned WJ-MSCs (WJ-MSCsHYP+APO)-derived secretome exhibited superior immunomodulatory activity, suppressing T-cell proliferation, enhancing Treg and Th2/Th9 differentiation, and promoting M2 macrophage polarization. It reduced mitochondrial ROS, improved mitochondrial polarization, and shifted T-cell metabolism from glycolysis to oxidative phosphorylation. Proteomic analysis revealed downregulation of IL-12, IL-17, and JAK–STAT signaling, along with modulation of complement, coagulation, and metabolic pathways. Secretome also enhanced extracellular matrix remodeling and antioxidant responses, supporting tissue repair. Dual preconditioning significantly amplifies the immunoregulatory, antioxidant, and regenerative efficacy of WJ-MSCs-derived secretome, presenting a promising non-cellular therapeutic approach for aGVHD management.
HostingRepository	PRIDE
AnnounceDate	2026-05-04
AnnouncementXML	Submission_2026-05-03_17:30:30.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ranjit Sahoo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-03 04:42:25	ID requested
⏵ 1	2026-05-03 17:30:30	announced

Dataset with its publication pending

submitter keyword: Immunomodulation, Antioxidant, Metabolic shift, Tissue repair, Apoptosis, Secretome, Mesenchymal Stem Cells,Acute Graft-versus-Host-Disease, Hypoxia

Prof. Ranjit Kumar Sahoo
contact affiliation	All India Institute of Medical Sciences, New Delhi, India
contact email	draranjitmd@gmail.com
lab head
Ranjit Sahoo
contact affiliation	All India Institute of Medical Sciences, New Delhi
contact email	drranjitmd@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD077957

PRIDE project URI

• PRIDE
  1. PXD077957
     1. Label: PRIDE project
     2. Name: Dual Preconditioned Mesenchymal Stem Cells-Derived Secretome Augment Immunomodulation and Drive Metabolic Reprogramming in Acute Graft-versus-Host Disease