PXD077924-1

PXD077924 is an original dataset announced via ProteomeXchange.

Title	Assembly of the catalytic module and the rotor of human ATP synthase.
Description	Human ATP synthase is a molecular rotary machine bound in inner mitochondrial membranes, built from twenty-eight subunits of seventeen kinds, two encoded in mitochondrial DNA, the remainder in nuclear genes. The machine consists of a rotor and an interacting stator. Turning of the rotor driven by a transmembrane proton motive force effects a cycle of structural changes in the catalytic part of the stator producing three ATP molecules per rotation. Here, to establish how the stator and rotor are assembled, we deleted subunits and known assembly factors from human cells, purified accumulated assembly intermediate complexes and characterized them by gel analysis and mass spectrometry, allowing us to propose pathways of assembly of the rotor and the catalytic F1-module of the stator. These observations provide opportunities for further development by structural analysis of the accumulated intermediates. The compositions of the various assembly intermediates support the view that ATP synthase arose via independent evolution of its three constituent structural components, the catalytic F1-module, the peripheral stalk module and the membrane associated Fo-module.
HostingRepository	PRIDE
AnnounceDate	2026-06-27
AnnouncementXML	Submission_2026-06-27_00:47:01.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ian M. Fearnley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-05-01 12:41:31	ID requested
⏵ 1	2026-06-27 00:47:02	announced

He J, Carroll J, Ding S, Li J, Fearnley IM, Walker JE, Assembly of the catalytic module and the rotor of human ATP synthase. EMBO J, ():(2026) [pubmed]

10.1038/s44318-026-00842-9;

submitter keyword: LC-MSMS,Human mitochondria/ATP synthase/assembly/F1-module/rotor

Prof John E. Walker
contact affiliation	Medical Research Council Mitochondrial Biology Unit, University of Cambridge. U.K.
contact email	jew30@cam.ac.uk
lab head
Ian M. Fearnley
contact affiliation	Medical Research Council Mitochondrial Biology Unit University of Cambridge
contact email	imf@mrc-mbu.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077924
     1. Label: PRIDE project
     2. Name: Assembly of the catalytic module and the rotor of human ATP synthase.