PXD077910 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions |
| Description | Virological research has traditionally focused on individual viruses or viral families. Advances in DNA synthesis now allow large-scale construction of individual gene products, enabling systematic exploration of the virome. Here, we developed a barcoded library of ~12,000 viral open reading frames (vORFs) from 513 viral species, which we leveraged to agnostically identify regulators of cellular proliferation and the innate and adaptive immune responses. Using this platform, we identified hundreds of viral regulators of cellular proliferation, class I MHC antigen presentation, and interferon signaling. Integrating results across these screens revealed unique phenotypic profiles and functional vORF modules, allowing the in-depth characterization of two previously uncharacterized viral proteins, MC162R and YLDV 151R, which impair class I MHC antigen presentation and IFN signaling, respectively. Together, the viral ORFeome provides a scalable framework for dissecting viral protein function across the breadth of the virome. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-07_04:21:34.837.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Colin O'Leary |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-05-01 03:21:34 | ID requested | |
| ⏵ 1 | 2026-09-07 04:21:35 | announced | |
Publication List
| 10.1016/j.cell.2026.05.024; |
| Fujimura E, O'Leary CN, Li MZ, Roberts RA, Glassman CR, Paulo JA, Jiang H, Abdelfattah NS, Wooten EC, Mirman Z, Harper JW, Cole PA, Elledge SJ, A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions. Cell, 189(17):5411-5428.e13(2026) [pubmed] |
Keyword List
| submitter keyword: YLDV 151R,Virome |
Contact List
| Stephen Elledge |
|---|
| contact affiliation | Department of Genetics, Harvard Medical School, Boston, MA, 02115, USA Division of Genetics, Brigham & Women's Hospital, Boston, MA 02115, USA Howard Hughes Medical Institute, Boston, MA 02115, USA |
| contact email | selledge@genetics.med.harvard.edu |
| lab head | |
| Colin O'Leary |
|---|
| contact affiliation | Division of Genetics, Brigham & Women's Hospital
Department of Genetics, Harvard Medical School |
| contact email | cno487@g.harvard.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD077910 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077910
- Label: PRIDE project
- Name: A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions
