PXD077831-1

PXD077831 is an original dataset announced via ProteomeXchange.

Title	The baseline vitreous proteome in rhegmatogenous retinal detachment: a case–control study of proliferative vitreoretinopathy
Description	Quantitative proteomic analysis of undiluted human vitreous biopsies collected at the time of primary surgical repair of rhegmatogenous retinal detachment (RRD), comparing patients who later developed proliferative vitreoretinopathy (PVR; n=8) with matched RRD controls who did not (n=8). Vitreous samples were processed by S-Trap digestion, labeled with TMT 10-plex reagents, and analyzed by LC–MS/MS on a Thermo Q Exactive instrument (two TMT 10-plex sets, two LC-MS/MS injection replicates per set; four .raw files total). Identification and quantitation were performed using Mascot followed by the proteoQ R pipeline. The dataset comprises 879 quantified proteins after stringent contaminant filtering. The deposition is intended as a methodologically transparent reference dataset of the baseline RRD vitreous proteome for future meta-analyses.
HostingRepository	PRIDE
AnnounceDate	2026-05-05
AnnouncementXML	Submission_2026-05-05_15:45:40.410.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rithwick Rajagopal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-29 20:22:25	ID requested
⏵ 1	2026-05-05 15:45:41	announced

Dataset with its publication pending

submitter keyword: PVR, TMT-10-plex,proteomics, vitreous, ocular, retinal detachment, biomarker, proliferative vitreoretinopathy

Rithwick Rajagopal, MD, PhD
contact affiliation	John F. Hardesty, MD Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, Saint Louis, Missouri, United States
contact email	rajagopalr@wustl.edu
lab head
Rithwick Rajagopal
contact affiliation	Washington University School of Medicine
contact email	rajagopalr@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077831
     1. Label: PRIDE project
     2. Name: The baseline vitreous proteome in rhegmatogenous retinal detachment: a case–control study of proliferative vitreoretinopathy