PXD077805 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of RRP12-containing complexes in HeLa cells upon RPS19 depletion |
| Description | This dataset contains a mass spectrometry-based proteomic analysis of RRP12-containing complexes isolated from human HeLa cells. The study was designed to analyze the interactome of the ribosome biogenesis factor RRP12 in the context of RPS19 deficiency, a defect associated with Diamond-Blackfan anemia. Complexes were purified from control and RPS19-knockdown HeLa cells (endogenously expressing GFP-RRP12) using the preribosome extraction and GFP-Trap immunoprecipitation protocol described by Singh et al. (2021; PMID: 34516797). Comparative quantitative analysis of the contents of RRP12-associated proteins, normalized to the amount of RRP12 in each sample, reveals an accumulation of large preribosomal complexes containing SSU-processome ribosome biogenesis factors (RBFs) upon RPS19 depletion. The description of these findings, together with the associated raw data analysis tables, are included in the article entitled "Convergent nuclear proteostasis alterations across ribosomal protein deficiencies" by Gaspar et al. (2026). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-26 |
| AnnouncementXML | Submission_2026-06-26_02:57:17.801.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD077805 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mercedes Dosil |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-29 11:11:38 | ID requested | |
| ⏵ 1 | 2026-06-26 02:57:18 | announced | |
Publication List
Keyword List
| submitter keyword: ribosome synthesis, preribosome, ribosomal protein deficiency, protein aggregates, nucleolus |
Contact List
| Mercedes Dosil |
|---|
| contact affiliation | Centro de Investigación del Cáncer, CSIC/Universidad de Salamanca, Spain |
| contact email | mdosil@usal.es |
| lab head | |
| Mercedes Dosil |
|---|
| contact affiliation | Centro de Investigación del Cáncer, Universidad de Salamanca |
| contact email | mdosil@usal.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077805
- Label: PRIDE project
- Name: Proteomic analysis of RRP12-containing complexes in HeLa cells upon RPS19 depletion
