⮝ Full datasets listing
PXD077743-1
PXD077743 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RNAs anchoring replication complex control initiation and firing of DNA replication Full submission |
| Description | Coordinated initiation of DNA replication is essential to ensure efficient and timely DNA synthesis. Yet the molecular determinants that confer origin selectivity in mammalian cells remain incompletely defined. Herein, we present data demonstrating a pivotal role for RNAs transcribed in the proximity of actively replicating gene loci. We show that RNAs aNChoring ORC1 (ANCORs) to the histone variant H2A.Z facilitate origin firing during DNA replication. This ANCOR-H2A.Z interaction appears to be essential for cells to duplicate their genetic material. Widespread and locus-specific perturbations of these transcripts correlate with anomalous replication patterns and a notable loss of the H2A.Z replicative marker at the origin site. Collectively, we present a previously undescribed RNA-mediated mechanism that is associated with the generation of active replication origins in mammalian cells. Our findings delineate a strategy to modulate the origins of replication in human cells at a local and global level, with potentially broad biomedical implications. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-28 |
| AnnouncementXML | Submission_2026-04-28_11:55:59.483.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Bogdan Budnik |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Deamidated |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-28 09:56:19 | ID requested | |
| ⏵ 1 | 2026-04-28 11:56:00 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: RNAs, DNA Replication, ORC1, H2A.Z, active replication origins, DatasetType:Proteomics |
Contact List
| Annalisa Diruscio | |
|---|---|
| contact affiliation | Beth Israel Deaconess Medical Center, Harvard Medical SChool |
| contact email | adirusci@bidmc.harvard.edu |
| lab head | |
| Bogdan Budnik | |
| contact affiliation | Harvard University |
| contact email | Bogdan.Budnik@wyss.harvard.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101636/ |
