⮝ Full datasets listing
PXD077679-2
PXD077679 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Simple Workflow for Integrated and Fast Tissue-preparation for microgram-level FF and FFPE Tissues Proteomics Analysis |
| Description | We present Simple Workflow for Integrated and Fast Tissue-preparation (SWIFT) workflows, enabling complete processing of fresh-frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) tissues in one- or two-step formats, while maintaining deep proteome coverage with good repeatability. For FF tissues, an incubation process integrating lysis, reduction, alkylation, and digestion can generate peptide samples directly from tissues in ≤ 1.5 h. For FFPE tissues, simultaneous deparaffinization, rehydration, and de-crosslinking was achieved within 0.5 h, followed by one-step pretreatment to obtain peptides. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-05-11_02:07:49.169.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chuping Wei |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-27 17:43:31 | ID requested | |
| 1 | 2026-04-30 01:10:08 | announced | |
| ⏵ 2 | 2026-05-11 02:07:49 | announced | 2026-05-11: Add info |
Publication List
| Wei C, Zhang Q, Fu C, Leng Y, Huang C, He F, Yang Y, Simple, Fast, and Highly Efficient One- or Two-Step Proteomic Preparation Enables Deep Profiling of Microgram-Level FF and FFPE Tissues. Anal Chem, 98(18):13397-13408(2026) [pubmed] |
Keyword List
| submitter keyword: Tissue proteomics, LC-MS, sample preparation, fresh frozen, formalin-fixed, paraffin-embedded, preservation method |
Contact List
| Yun Yang | |
|---|---|
| contact affiliation | International Academy of Phronesis Medicine (Guang Dong) |
| contact email | yangyunwww@126.com |
| lab head | |
| Chuping Wei | |
| contact affiliation | Southern University of Science and Technology |
| contact email | 1650551723@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
