⮝ Full datasets listing
PXD077678-1
PXD077678 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A detergent-free and desalting-free sample preparation method for tandem-enrichment of muiltiple PTMs |
| Description | Mass spectrometry (MS)-based proteomics requires efficient sample preparation for post-translational modification (PTM) analysis. Here, we present a detergent- and desalting-free method (DFSP) enabling tandem enrichment of ubiquitination, phosphorylation, and glycosylation. DFSP employs a two-step digestion: initial tryptic cleavage under native conditions followed by heat-induced denaturation (95°C, 10 min), achieving proteolytic efficiency comparable to detergent-based methods (75,974 peptides identified). By eliminating desalting steps, DFSP directly enriches PTM peptides using K-GG antibody beads, CaTiO3, and ZIC-HILIC, yielding 10,130 ubiquitinated peptides, 47,057 phosphopeptides, and 700 glycopeptides from 500 μg HeLa proteins. Integrated with diaPASEF-MS and computational platforms (FragPipe/DIA-NN), DFSP revealed IFNγ-induced regulation of 18,584 phosphosites and 2,117 glycosites, with high reproducibility (Pearson correlation >0.8). This streamlined workflow reduces processing time and sample loss, offering a robust tool for multi-PTM profiling in complex biological systems. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-04-27_17:39:58.919.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chuanqi Zhong |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-27 17:39:40 | ID requested | |
| ⏵ 1 | 2026-04-27 17:39:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: detergent-free, post-translational modification, phosphorylation, ubiquitination, glycosylation |
Contact List
| Chuanqi Zhong | |
|---|---|
| contact affiliation | Xiamen University |
| contact email | zhongcq@xmu.edu.cn |
| lab head | |
| Chuanqi Zhong | |
| contact affiliation | Xiamen Univeristy |
| contact email | zhongcq@xmu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
