⮝ Full datasets listing
PXD077644-1
PXD077644 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | XL-MS analysis of COMPASS complex and its interactions with nucleosome |
| Description | This project presents cross-linking mass spectrometry (XL-MS) data of the COMPASS complex from Saccharomyces cerevisiae. COMPASS (Complex Proteins Associated with Set1) is a conserved histone H3 lysine 4 (H3K4) methyltransferase essential for transcriptional regulation, cell cycle progression, and chromatin signaling. The Cps35 subunit (also known as Swd2) is a critical component for COMPASS stability and its interaction with methylated H3K4. To dissect the architectural organization and the role of Cps35 in COMPASS-nucleosome interaction, we performed XL-MS analysis on four distinct conditions: (1) intact COMPASS complex, (2) COMPASS lacking the Cps35 subunit (COMPASS Δcps35), (3) COMPASS bound to nucleosome, and (4) COMPASS Δcps35 bound to nucleosome. All protein complexes were overexpressed in yeast and purified via TAP-tag affinity purification. This dataset includes raw MS files, search engine outputs, and identified cross-linked peptides for each condition .These data provide structural insights into the subunit interaction network of COMPASS and the molecular role of Cps35 in mediating COMPASS-nucleosome engagement. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-26 |
| AnnouncementXML | Submission_2026-04-27_01:31:33.134.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qin Hong |
| SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-27 01:31:12 | ID requested | |
| ⏵ 1 | 2026-04-27 01:31:33 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Cross-linking mass spectrometry，BS3 |
Contact List
| Yao Cong | |
|---|---|
| contact affiliation | Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences |
| contact email | cong@sibcb.ac.cn |
| lab head | |
| Qin Hong | |
| contact affiliation | Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences |
| contact email | hongqin2018@sibcb.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
