PXD077599-1

PXD077599 is an original dataset announced via ProteomeXchange.

Title	Exercise-Associated Microbial Metabolites Prevent Skeletal Muscle Atrophy in Adult Mice
Description	We previously reported that skeletal muscle adaptation to regular exercise requires a healthy gut microbiome, contributing to growing evidence that some exercise benefits are mediated by microbiome-derived metabolites. Here, to identify such exercise-associated microbial metabolites, we transfer cecal contents from exercise-trained donor mice into exercise-naïve recipient mice undergoing unilateral hindlimb immobilization. Recipients of cecal material from exercise-trained donors exhibit less muscle atrophy compared with those receiving transfers from sedentary donors. Untargeted metabolomics reveal metabolites enriched in cecal content, serum, and muscle of recipients from exercise-trained donors, consistent with microbial origin. Oral administration of two such metabolites (pipecolic acid and succinate) attenuates muscle atrophy and preserves muscle function in exercise-naïve mice, potentially by enhancing cellular energy status and translational capacity. These findings further define the gut microbiome-skeletal muscle axis and provide evidence that exercise-associated microbial metabolites serve as a novel class of exercise mimetics for treating conditions responsive to physical activity.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_10:38:56.576.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuan Wen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-25 00:02:43	ID requested
⏵ 1	2026-05-27 10:38:57	announced

Dataset with its publication pending

submitter keyword: Mouse Skeletal Muscle Proteomics

John Joseph McCarthy
contact affiliation	Department of Physiology, University of Kentucky, USA
contact email	jjmcca2@uky.edu
lab head
Yuan Wen
contact affiliation	University of Kentucky
contact email	ywen2@uky.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077599
     1. Label: PRIDE project
     2. Name: Exercise-Associated Microbial Metabolites Prevent Skeletal Muscle Atrophy in Adult Mice