PXD077571-1

PXD077571 is an original dataset announced via ProteomeXchange.

Title	Longitudinal quality control proteomics dataset of 293T cells acquired on an Orbitrap Astral mass spectrometer for Apus workflow validation
Description	This dataset was generated to validate the real-time quality control and automated data analysis capabilities of Apus, an end-to-end solution for large-cohort mass spectrometry-based proteomics. The dataset consists of 23 longitudinally acquired data-dependent acquisition (DDA) runs from HEK 293T cell digest samples, prepared and analyzed under routine laboratory operating conditions on an Orbitrap Astral mass spectrometer (Thermo Scientific) coupled to a Vanquish Neo nanoflow LC system. The dataset was processed through the complete Apus automated workflow, including real-time data aggregation (ApuPickup), parallelized protein identification (ApuPioneer), high-throughput label-free quantification (ApuHorizon), and comprehensive quality control (ApuMonitor), to demonstrate the system's capability for proactive quality management in a live laboratory setting.
HostingRepository	iProX
AnnounceDate	2026-04-24
AnnouncementXML	Submission_2026-04-24_00:11:43.420.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zekun Cai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-24 00:10:53	ID requested
⏵ 1	2026-04-24 00:11:43	announced

Dataset with its publication pending

submitter keyword: Human, 293T, DDA, LC-MS/MS, Orbitrap Astral, Quality control, Proteomics, Longitudinal

Chao Liu
contact affiliation	Beihang University
contact email	liuchaobuaa@buaa.edu.cn
lab head
Zekun Cai
contact affiliation	Beihang University
contact email	zekun.cai@outlook.com
dataset submitter

iProX dataset URI