PXD077526-1

PXD077526 is an original dataset announced via ProteomeXchange.

Title	In vitro PhoX cross-linking of purified hsMic60
Description	Mitochondrial crista junctions (CJs) operate as regulated gateways into the cristae microenvironment, whose protein, metabolite, and ion compositions are finely tuned for mitochondrial function. The Mic60-Mic19 complex of the mitochondrial contact site and cristae organizing system (MICOS) complex was suggested to span across CJs and act as a diffusion barrier, but little is known of how its dynamic architecture facilitates this task. To address this open question, we determined the crystal structure of an amino-terminal dimeric helical bundle of human Mic60. These and previous structural and biochemical data were harnessed in molecular dynamic (MD) simulations to develop a dynamic model of the human tetrameric Mic60-Mic19 subcomplex in the CJ environment, to validate its architecture using in organello cross-linking data and to computationally characterize its function as a diffusion barrier. Our integrative structural biology approach enables the functional investigation of flexible, multidomain protein complexes which escape conventional structural biology methods. This in vitro PhoX cross-linking mass spectrometry data of purified hsMic60 supports the in organello studies by showing a similar cross-linking pattern.
HostingRepository	PRIDE
AnnounceDate	2026-08-04
AnnouncementXML	Submission_2026-08-04_03:06:18.506.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Max Ruwolt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-23 04:51:08	ID requested
⏵ 1	2026-08-04 03:06:18	announced

Dataset with its publication pending

submitter keyword: MICOS, cross-linking,mitochondria, XL-MS, PhoX

Oliver Daumke
contact affiliation	Max-Delbrück-Centrum für Molekulare Medizin (MDC) Robert-Rössle-Straße 10 13125 Berlin, Germany
contact email	oliver.daumke@mdc-berlin.de
lab head
Max Ruwolt
contact affiliation	Protein Biochemistry Institute for Chemistry and Biochemistry Freie Universität Berlin
contact email	ruwolt.max@fu-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077526
     1. Label: PRIDE project
     2. Name: In vitro PhoX cross-linking of purified hsMic60