⮝ Full datasets listing
PXD077456-1
PXD077456 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Integrated Multi-Omics and Experimental Validation Reveal Dysregulation of the OXPHOS-NADPH-GSH Axis in Renal Fibrosis |
| Description | This project explores the metabolic mechanisms linking mitochondrial dysfunction to redox imbalance during the progression of renal fibrosis in Chronic Renal Failure (CRF). Using an integrated multi-omics approach, we performed TMT-based quantitative proteomics and LC-MS/MS untargeted metabolomics on fibrotic renal tissues to map the molecular landscape of fibrogenesis. Our data reveals a significant remodeling of mitochondrial oxidative phosphorylation (OXPHOS) components alongside the downregulation of critical NADPH-generating enzymes, specifically ME1, ME2, and IDH1. These proteomic shifts correlate with metabolomic evidence of decreased NADPH availability and a disrupted glutathione redox system (GSH/GSSG). Furthermore, we identified the suppression of NRF2-dependent antioxidant defenses, including HO-1 and GPX4, which drives excessive oxidative stress and extracellular matrix accumulation. The study further validates these findings through in vitro experiments, demonstrating that restoring redox homeostasis with N-acetylcysteine (NAC) or Mito-TEMPO alleviates TGF-β1-induced fibroblast activation. This dataset provides comprehensive evidence that the coordinated disruption of mitochondrial OXPHOS and NADPH metabolism is a central driver of renal fibrosis. By archiving these proteomic and metabolomic profiles, we provide a valuable resource for identifying therapeutic targets focused on mitochondrial redox metabolism to mitigate the global burden of chronic kidney disease and fibrotic progression. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-21 |
| AnnouncementXML | Submission_2026-08-31_20:18:48.551.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dongdong Wu |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-21 18:36:50 | ID requested | |
| ⏵ 1 | 2026-08-31 20:18:49 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: chronic renal failure, renal fibrosis, oxidative phosphorylation |
Contact List
| Shouzhu Xu | |
|---|---|
| contact affiliation | Department of Public Health, Shaanxi University of Chinese Medicine |
| contact email | xsz@sntcm.edu.cn |
| lab head | |
| Dongdong Wu | |
| contact affiliation | Department of Public Health, Shaanxi University of Chinese Medicine |
| contact email | wdd7514@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
