PXD077447 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of novel substrates reveals a role for DYRK1B in regulating processing body abundance |
| Description | Dual-specificity tyrosine-phosphorylation-regulated kinase 1B (DYRK1B) modulates the cell cycle, cell fate during development, and is deregulated in cancer and metabolic syndrome. However, only a few substrates of DYRK1B have been defined. Therefore, we sought to fill this gap in knowledge combining quantitative phosphoproteomics in DYRK1B-inducible cell lines. Motif analysis revealed enrichment for proline-directed serine or threonine phosphorylation sites (pSer/pThr-Pro), consistent with the consensus motif of class I DYRKs. Gene ontology analysis showed enrichment of proteins involved in mRNA binding, mRNA processing and ribonucleoprotein complexes. Several processing body (PB) components, including DCP1A, PATL1(PAT1B), EDC3 and 4E-T, were identified as DYRK1B-inducible phosphoproteins. DYRK1B also co-immunoprecipitated with DCP1A, PAT1B, EDC3, EDC4, DDX6 and XRN1. Super-resolution microscopy demonstrated that DYRK1B co-localised with DCP1A, DCP1B and DDX6 in PBs. Activation of DYRK1B increased PB abundance, whereas inhibition, depletion or knockout of DYRK1B reduced phosphorylation of DCP1A and 4E-T and decreased PB number. Re-expression of wild-type, but not kinase-dead, DYRK1B restored PB numbers in knockout cells. These findings reveal novel DYRK1B targets and establish DYRK1B as a regulator of processing body dynamics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-02 |
| AnnouncementXML | Submission_2026-09-02_06:48:01.922.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lu Yu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-21 12:37:10 | ID requested | |
| ⏵ 1 | 2026-09-02 06:48:02 | announced | |
Publication List
Keyword List
| submitter keyword: DYRK1B, p-SILAC,DCP1A, processing bodies, condensates |
Contact List
| David Oxley |
|---|
| contact affiliation | Babraham Institute |
| contact email | David.Oxley@Babraham.ac.uk |
| lab head | |
| Lu Yu |
|---|
| contact affiliation | Babraham Institute |
| contact email | lu.yu@babraham.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077447
- Label: PRIDE project
- Name: Identification of novel substrates reveals a role for DYRK1B in regulating processing body abundance
