⮝ Full datasets listing
PXD077428-1
PXD077428 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Site-specific Profiling of Lysine Itaconylation via a Thiol-based Probe |
| Description | Lysine itaconylation is a novel post-translational modification (PTM) that was recently discovered in macrophage proteomes mediated by the immunoregulatory metabolite, itaconate. However, there is currently an absence of comprehensive and high-accuracy analytical methods for the global identification of itaconylation sites in proteomes, which limits its biological function study. Here, we developed a thiol-based bioorthogonal probe, BMAyne probe, to site-specifically profile itaconylation in macrophage proteomes by an Activity-based Protein Profiling (ABPP) strategy. Notably, 31 endogenous itaconylation sites on 29 proteins were identified in lipopolysaccharide (LPS)-stimulated macrophages using a thiol-based probe, BMAyne probe, which complemented the results obtained by previous promiscuous antibody enrichment. Our effort provides a unique chemical tool to enrich endogenous lysine itaconylation and establishes a rich database for guiding subsequent functional studies of this unique lysine itaconylation PTM. |
| HostingRepository | iProX |
| AnnounceDate | 2026-04-21 |
| AnnouncementXML | Submission_2026-04-21_02:29:37.111.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ziming Tang |
| SpeciesList | scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-21 02:28:52 | ID requested | |
| ⏵ 1 | 2026-04-21 02:29:37 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Itaconate, Lysine itaconylation, ABPP, Chemoproteomics, thiol probe |
Contact List
| Chu Wang | |
|---|---|
| contact affiliation | Peking University |
| contact email | chuwang@pku.edu.cn |
| lab head | |
| Ziming Tang | |
| contact affiliation | Peking University |
| contact email | zmtang25@stu.pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
