PXD077415-1

PXD077415 is an original dataset announced via ProteomeXchange.

Title	An Exploratory Proteomics Study of Autoimmune Premature Ovarian Insufficiency in a Mouse Model
Description	Premature ovarian insufficiency (POI) impairs fertility and health in reproductive-age women, with autoimmune factors contributing to 4-30% of cases[1]. To investigate immune dysregulation in POI, we developed two mouse models using pZP3 induction: regular immune (RE-POI) and enhanced immune (EN-POI) cycles. The EN-POI model exhibited stable, irreversible ovarian dysfunction, including disrupted estrous cycles, hormonal changes (elevated FSH, decreased AMH and estradiol), follicular depletion, and infertility. Immune profiling revealed increased CD4+ T cells and decreased regulatory T cells in the spleen, elevated inflammatory cytokines (e.g., interferon-γ, CCL4, CCL5) in serum, and ovarian fibrosis. Proteomics identified 198 differentially expressed proteins enriched in immune and inflammatory pathways. Network analysis highlighted six hub proteins, such as MMP9, ISG15, and IKBKE, with expressions negatively correlating with ovarian reserve markers in human POI. This study establishes a stable autoimmune POI model, elucidates T-cell imbalance with cytokine storm and fibrosis, and identifies key molecules linking immune abnormalities to ovarian failure, offering new insights for POI research.
HostingRepository	iProX
AnnounceDate	2026-04-20
AnnouncementXML	Submission_2026-04-20_19:35:55.675.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Tian
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-20 19:35:27	ID requested
⏵ 1	2026-04-20 19:35:56	announced

Dataset with its publication pending

submitter keyword: premature ovarian insufficiency,autoimmune,mouse model,proteomics,immune imbalance

Feiyang Diao
contact affiliation	Nanjing Medical University
contact email	diaofeiyang@njmu.edu.cn
lab head
Ying Tian
contact affiliation	Nanjing Medical University
contact email	tianying@stu.njmu.edu.cn
dataset submitter

iProX dataset URI