PXD077389-1

PXD077389 is an original dataset announced via ProteomeXchange.

Title	Identification of enzymes involved in negamycin production
Description	Negamycin is a hydrazide-containing antibiotic characterized by an unusual N–N bond. It exhibits broad-spectrum activity against both Gram-positive and Gram-negative bacteria by inhibiting translation. In addition, its ability to induce stop codon read-through makes it a promising therapeutic candidate for the treatment of genetic diseases caused by premature termination codons. Negamycin is produced by the actinomycete Kitasatospora purpeofusca, yet its biosynthetic gene cluster and biosynthetic pathway remain uncharacterized. To uncover the molecular basis of negamycin biosynthesis, we employ comparative proteomics by analyzing differences between the wild-type strain and a negamycin-deficient mutant, as well es negamycin heterologous host S. albidovlavus Del14 containing negamycin biosynthetic genes. This approach aims to identify proteins potentially involved in the biosynthetic process.
HostingRepository	PRIDE
AnnounceDate	2026-09-10
AnnouncementXML	Submission_2026-09-10_03:12:07.898.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mirita Franz-Wachtel
SpeciesList	scientific name: Kitasatospora purpeofusca; NCBI TaxID: NEWT:67352; scientific name: Streptomyces albidoflavus; NCBI TaxID: NEWT:1886;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-20 10:45:10	ID requested
⏵ 1	2026-09-10 03:12:08	announced

Dataset with its publication pending

submitter keyword: BGC, N-N bond formation, Actinomycetes,Negamycin, Proteomics

Evi Stegmann
contact affiliation	Interfaculty Institute for Microbiology and Infection Medicine Tübingen Eberhard-Karls Universität Tübingen Auf der Morgenstelle 28 72072 Tübingen
contact email	evi.stegmann@uni-tuebingen.de
lab head
Mirita Franz-Wachtel
contact affiliation	Proteome Center Tuebingen
contact email	mirita.franz@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD077389

PRIDE project URI

• PRIDE
  1. PXD077389
     1. Label: PRIDE project
     2. Name: Identification of enzymes involved in negamycin production