PXD077329-1

PXD077329 is an original dataset announced via ProteomeXchange.

Title	Complement proteins enriched in dextran-based microparticle
Description	Autoimmunity affects ~10% of the global population. Current therapies suppress protective immunity and are non-curative. Antigen-specific therapies can selectively suppress autoreactive cells, preserving protective immunity. B cell dysregulation, a major driver of autoimmunity, remains largely untargeted. We hypothesized that targeting B cells with antigen-loaded microparticles enhances therapeutic efficacy. Here, we demonstrate that antigen-loaded acetalated dextran microparticles (AMP) surface-associate with B cells and enhance IL-10 secretion and MHCII expression, promoting tolerogenic antigen presentation. Leveraging this property, we developed a therapy using myelin oligodendrocyte (MOG35-55) peptide-loaded AMPs associated with B cells (MOG-AMP-B). Administering MOG-AMP-Bs in a late therapeutic model of multiple sclerosis resulted in unprecedented recovery, reducing central nervous system (CNS) inflammation, downregulating activated dendritic cells and macrophages and increasing regulatory T- and B cells. MOG-AMP-Bs did not reduce the ability of animals to respond to a viral infection, representing a highly effective antigen-specific therapy for restoring immune balance in autoimmunity. This study includes proteomics on the AMP particles after serum exposure, identifying complement proteins enriched on its protein corona.
HostingRepository	PRIDE
AnnounceDate	2026-05-19
AnnouncementXML	Submission_2026-05-19_07:14:33.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott Lyons
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-17 13:13:38	ID requested
⏵ 1	2026-05-19 07:14:34	announced

Dataset with its publication pending

submitter keyword: protein corona, microparticle,complement, dextran

Kristy Ainslie
contact affiliation	Division of Pharmacoengineering and Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill
contact email	ainsliek@email.unc.edu
lab head
Scott Lyons
contact affiliation	UNC Chapel Hill
contact email	scott_lyons@med.unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077329
     1. Label: PRIDE project
     2. Name: Complement proteins enriched in dextran-based microparticle