PXD077311 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of PPARα-associated proteins in mouse macrophages by immunoprecipitation coupled with Q Exactive LC-MS/MS |
| Description | This PRIDE project contains raw and processed LC-MS/MS data generated from immunoprecipitation–mass spectrometry (IP-MS) experiments designed to identify PPARα-associated proteins in mouse bone marrow-derived macrophages (BMDMs). In this study, His-tagged PPARα was overexpressed in BMDMs and immunoprecipitated using anti-His magnetic beads, followed by proteolytic digestion of the immunoprecipitated protein complexes and LC-MS/MS analysis. Peptides were separated using an Easy nLC 1200 nano-flow liquid chromatography system and analyzed on a Q Exactive HF-X mass spectrometer operated in data-dependent acquisition mode. Raw MS data were searched using MaxQuant (version 2.4.14.0). This dataset was generated as part of a study investigating the mechanism by which fibrin stiffness regulates macrophage polarization and metabolic reprogramming in metabolic dysfunction-associated steatohepatitis (MASH). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-09 |
| AnnouncementXML | Submission_2026-09-09_01:48:20.395.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | yan 宋岩 |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-17 08:30:49 | ID requested | |
| ⏵ 1 | 2026-09-09 01:48:21 | announced | |
Publication List
| 10.1007/s00018-026-06266-2; |
| Zhang R, Liu J, Shan W, Wang H, Gao L, Li Y, Fan L, Yang C, Li G, Liu J, -oxidation to alleviate MASH. Cell Mol Life Sci, 83(1):(2026) [pubmed] |
Keyword List
| submitter keyword: PPARα |
| Mus musculus |
| bone marrow-derived macrophages |
| affinity purification-mass spectrometry |
| immunoprecipitation |
Contact List
| Jing Liu |
|---|
| contact affiliation | Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China |
| contact email | liujing_GI@whu.edu.cn |
| lab head | |
| yan 宋岩 |
|---|
| contact affiliation | 山东医药大学 |
| contact email | zzyxicu1@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD077311 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077311
- Label: PRIDE project
- Name: Identification of PPARα-associated proteins in mouse macrophages by immunoprecipitation coupled with Q Exactive LC-MS/MS
