PXD077187-1

PXD077187 is an original dataset announced via ProteomeXchange.

Title	Establishment and application of a vesicle extraction method for clinical strains of Pseudomonas aeruginosa
Description	Pseudomonas aeruginosa is a versatile pathogen capable of causing illnesses that range from mild infections to life-threatening conditions. Its virulence is driven by a wide array of factors, among which extracellular vesicles (EVs) have gained recognition as important contributors to its pathogenicity. Despite this, the full scope of their roles remains unclear. A major barrier to EV characterization is the difficulty of vesicle isolation—procedures are often lengthy, yield is low, and specialized equipment is required. In this study, we assessed the effectiveness of a rapid vesicle extraction method from clinical strains of P. aeruginosa. To that end, we first selected and characterized six phenotypically diverse clinical strains of P. aeruginosa (two reference strains and 4 clinical isolates, including one strain from a cystic fibrosis patient) and used them to evaluate the vesicle extraction method. The results obtained through SDS-PAGE analysis, western blot, protein quantification, TEM, dynamic light scattering and mass spectrometry indicated the presence of vesicles in all samples; however, it was also possible to observe a large number of contaminants in some of them (mainly LS07 and Z37). Subsequent treatment with enzymes (DNase and/or alginate lyase) allowed for the elimination of the contaminants as observed by electron microscopy. Our results suggest that the method is suited for the vesicle extraction of clinical isolates of P. aeruginosa, where traditional methods, such as ultracentrifugation, are not available The phenotypic complexity of these strains presents challenges that current rapid purification methods are ill-equipped to handle, highlighting the need for improved or alternative approaches.
HostingRepository	PRIDE
AnnounceDate	2026-04-16
AnnouncementXML	Submission_2026-04-16_00:40:30.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Serena Schwenkert
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: NCBITaxon:287;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-15 07:32:05	ID requested
⏵ 1	2026-04-16 00:40:31	announced

Dataset with its publication pending

submitter keyword: None

Serena Schwenkert
contact affiliation	MSBioLMU Großhaderner Str. 2-4 82152 Planegg-Martinsried
contact email	serena.schwenkert@lmu.de
lab head
Serena Schwenkert
contact affiliation	LMU Munich
contact email	serena.schwenkert@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD077187
     1. Label: PRIDE project
     2. Name: Establishment and application of a vesicle extraction method for clinical strains of Pseudomonas aeruginosa